Investigation of drought induced biochemical and gene expression changes in carrot cultivars

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dc.authoridGOKCE, ALI FUAT/0000-0002-2953-0326
dc.authoridJunaid, Muhammad Daniyal/0000-0003-0982-9264
dc.authoridOZTURK, ZAHIDE NESLIHAN/0000-0002-2592-9997
dc.contributor.authorJunaid, Muhammad Daniyal
dc.contributor.authorGokce, Zahide Neslihan Ozturk
dc.contributor.authorGokce, Ali Fuat
dc.date.accessioned2024-11-07T13:24:09Z
dc.date.available2024-11-07T13:24:09Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground Carrot is the most important vegetable in Apiaceae family, and it is consumed globally due to its high nutritional quality. Drought stress is major environmental constraint for vegetables especially carrot. Limited data is available regarding the mechanisms conferring drought tolerance in carrot. Methods and Results Eight commercial carrot cultivars were used in this study and subjected to drought stress under semi-controlled greenhouse conditions. Biochemical, antioxidant enzymatic activity and changes in transcript level of drought related genes was estimated, the gene expression analysis was done by using qRT-PCR in comparison with reference gene expression Actin (Act1). Results revealed that cultivars Coral Orange, Tendersweet and Solar Yellow were tolerant to drought stress, which was supported by their higher transcript levels of catalase gene (CAT), superoxide dismutase genes (Cu/ZN-SOD, Cu/Zn-SDC) in these cultivars. The downregulation of PDH1 gene (Proline dehydrogenase 1) was also observed that was associated with upregulation of proline accumulation in carrot plants. Moreover, results also suggested that PRT genes (Proline transporter genes) played a key role in drought tolerance in carrot cultivars. Conclusion Among the cultivars studied, Coral Orange showed overall tolerance to drought stress conditions, whereas cultivars Cosmic Purple and Eregli Black were sensitive based on their biochemical and gene expression levels. According to our knowledge, this is the first comparative study on drought tolerance in several carrot cultivars. It will provide a background for carrot breeding to understand biochemical and molecular responses of carrot plant to drought stress and mechanisms behind it.
dc.description.sponsorshipScientific Research Projects Unit (BAP) of Nigde Omer Halisdemir University, Nigde, Turkiye [TGT 2020/14-BAGEP]
dc.description.sponsorshipScientific Research Projects Unit (BAP) of Nigde Omer Halisdemir University, Nigde, Turkiye under the Project No. TGT 2020/14-BAGEP.
dc.identifier.doi10.1007/s11033-022-08050-4
dc.identifier.endpage359
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1
dc.identifier.pmid36331749
dc.identifier.scopus2-s2.0-85141369693
dc.identifier.scopusqualityQ2
dc.identifier.startpage349
dc.identifier.urihttps://doi.org/10.1007/s11033-022-08050-4
dc.identifier.urihttps://hdl.handle.net/11480/13951
dc.identifier.volume50
dc.identifier.wosWOS:000878965800007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectDaucus carota L
dc.subjectProline transporters
dc.subjectProline dehydrogenase
dc.subjectSuperoxide dismutase
dc.subjectDrought stress
dc.subjectBiochemical analyses
dc.titleInvestigation of drought induced biochemical and gene expression changes in carrot cultivars
dc.typeArticle

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