Isolation, genotyping and antimicrobial susceptibility of pathogenic Escherichia coli serotypes in ready to eat foods

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dc.authoridHIZLISOY, Harun/0000-0003-3391-0185
dc.authoridKaradal, Fulden/0000-0002-5113-5883
dc.authoridERTAS ONMAZ, Nurhan/0000-0002-4679-6548
dc.authoridAL, Serhat/0000-0003-2721-9275
dc.contributor.authorKaradal, F.
dc.contributor.authorOnmaz, N. Ertas
dc.contributor.authorHizlisoy, H.
dc.contributor.authorAl, S.
dc.contributor.authorTelli, N.
dc.contributor.authorYildirim, Y.
dc.contributor.authorGonulalan, Z.
dc.date.accessioned2024-11-07T13:32:36Z
dc.date.available2024-11-07T13:32:36Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractIn this study, pathogenic Escherichia coli serotypes (E. coli O157:H7, O26, O111) and their molecular proximity and antimicrobial susceptibility were investigated in RTE foods. A total of 240 samples; consist of 105 stuffed mussel, 56 meatless cig kofte, 54 Russian salad, 25 cheese halva, were analyzed. The conventional culture and serotyping methods for determination of the organisms were performed and further confirmation by PCR was carried out. Confirmed E. coli O157 isolates were genotyped by the enterobacterial repetitive intergenic consensus (ERIC)-PCR. Antibacterial susceptibility testing of the isolates was performed by disc diffusion method. E. coli was detected in 7 (2.9 %) of 240 samples, including 3 (5.5%) Russian salad, 3 (2.8%) stuffed mussel, 1 (4 %) cheese halva. Two isolates from Russian salad, 1 from stuffed mussel and 1 from cheese halva were identified as E. coli O157. In addition, stuffed mussel isolate was found to carry stx1 ve hlyA genes whereas one Russian salad isolate carried the stx1 gene. E. coli isolates were found to be resistant to amoxycillin/clavulonic acid, gentamicin and ciprofloxacin, at the rate of 29%, 14% and 29 %, respectively. Only one (14 %) isolate from stuffed mussel was classified as multidrug resistant to three antimicrobials. Furthermore, the isolates, related to O157 and O157:H7, presented different ribotypes in this study. The results provide useful data for the development of public health policy concerning the potential presence of pathogenic antimicrobial resistant E. coli serotypes in RTE foods. Strict surveillance of RTE foods at retail points for emerging pathogens, their antimicrobial resistance patterns and the potential likelihood of cross-contamination is required.
dc.identifier.endpage1668
dc.identifier.issn1792-2720
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85078105286
dc.identifier.scopusqualityQ3
dc.identifier.startpage1661
dc.identifier.urihttps://hdl.handle.net/11480/15515
dc.identifier.volume70
dc.identifier.wosWOS:000493815300010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherHellenic Veterinary Medical Soc
dc.relation.ispartofJournal of the Hellenic Veterinary Medical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectAntimicrobial susceptibility
dc.subjectcheese halva
dc.subjectERIC-PCR
dc.subjectmeatless cig kofte
dc.subjectRussian salad
dc.subjectEHEC
dc.subjectstuffed mussel
dc.titleIsolation, genotyping and antimicrobial susceptibility of pathogenic Escherichia coli serotypes in ready to eat foods
dc.typeArticle

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