Effects of different genotypes and gamma ray doses on haploidization with irradiated pollen technique in watermelon (Citrullus Ianatus L.)

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dc.contributor.authorTaskin, Hatira
dc.contributor.authorYucel, Namik Kemal
dc.contributor.authorBaktemur, Gokhan
dc.contributor.authorComlekcioglu, Songul
dc.contributor.authorBuyukalaca, Saadet
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2013
dc.departmentNiğde ÖHÜ
dc.description.abstractTwo watermelon genotypes, one commercial watermelon variety (Ustun F1) and five different doses of gamma rays coming from Co-60 were tested to develop useful haploidization procedures in watermelon. For this purpose, male flowers collected a day before anthesis were irradiated with 50, 150, 200, 275 and 300 Gy doses of gamma rays, and female flowers were pollinated with irradiated pollen the next day. Seeds extracted from fruits harvested 25 d later were opened individually in a laminar flow hood. Embryos obtained via embryo rescue technique were placed in glass tubes containing CP medium with 30 g L-1 sucrose, 8 g L-1 agar, 0.08 mg L-1 B12, and 0.02 mg L-1 IAA. Sixty haploid embryos were obtained from 43 watermelon fruits in this study. Genotype 1 was found to be the most successful genotype with 3.57 haploid embryos per 100 seeds. Among tested irradiation doses, 275 Gy was better than other doses, with 5.26 haploid embryos per 100 seeds. Considered together with irradiation dose and genotypes, the maximum number of haploid embryos was obtained from Genotype 1 pollinated with 275 Gy irradiation dose, with 6.25 haploid embryos per 100 seeds.
dc.description.sponsorshipRijk Zwaan Company; Cukurova University, Scientific Research Projects Coordinating Office [CU-BAP-ZF2011BAP24]
dc.description.sponsorshipThe authors want to thank the Rijk Zwaan Company and the Cukurova University, Scientific Research Projects Coordinating Office (CU-BAP-ZF2011BAP24) for supporting this study.
dc.identifier.doi10.4141/CJPS2013-059
dc.identifier.endpage1168
dc.identifier.issn0008-4220
dc.identifier.issn1918-1833
dc.identifier.issue6
dc.identifier.scopus2-s2.0-84887287395
dc.identifier.scopusqualityQ2
dc.identifier.startpage1165
dc.identifier.urihttps://dx.doi.org/10.4141/CJPS2013-059
dc.identifier.urihttps://hdl.handle.net/11480/4329
dc.identifier.volume93
dc.identifier.wosWOS:000328800400020
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherCANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
dc.relation.ispartofCANADIAN JOURNAL OF PLANT SCIENCE
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCitrullus lanatus
dc.subjectwatermelon
dc.subjecthomozygosity
dc.subjecthaploid embryo
dc.subjectirradiation dose
dc.titleEffects of different genotypes and gamma ray doses on haploidization with irradiated pollen technique in watermelon (Citrullus Ianatus L.)
dc.typeArticle

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