Stacked insecticidal genes in potatoes exhibit enhanced toxicity against Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

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dc.authoridGokce, Ayhan/0000-0002-5617-1349
dc.authoridSalim, Muhammad/0000-0003-3140-1299
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.contributor.authorSalim, Muhammad
dc.contributor.authorBakhsh, Allah
dc.contributor.authorGokce, Ayhan
dc.date.accessioned2024-11-07T13:24:45Z
dc.date.available2024-11-07T13:24:45Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe present study was performed to express stacked insecticidal genes in potato cv. Lady Olympia and Agria to encode resistance against Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say). Bacillus thuringiensis (Bt) gene (cry3A), synthetic hybrid (SN-19) and plant proteinase inhibitor Oryza cystatin II (OCII) cloned in pCAMBIA1301 binary vector in two different combinations as of DS-1 (cry3A + SN-19 genes) and DS-2 (OCII + SN-19 genes) constructs and further transformed to two potato cultivars using Agrobacterium-mediated transformation. All molecular analyses confirmed gene integration and expression in a total of 27 primary transformants in both Agria and Lady Olympia. Insecticidal effects of T0 progeny transgenic potato plants were tested against CPB under laboratory conditions. Transgenic plants of Agria and Lady Olympia transformed with DS-1 and DS-2 constructs caused 100% mortality to all larval stages and adults of CPB. However, 100% mortality of tested insects took a longer time in the adult stage (10-14 days) compared to larval stages (2-6 days). Foliage consumption by L2-L4 larval stages and adults of CPB was significantly reduced in Agria and Lady Olympia plants transformed with DS-1 and DS-2 constructs, as compared to their control plants. Lower foliage consumption of transgenic plants by L1 larval stages was also observed, but the reduction was only statistically significant for some of the tested plants. These promising results indicate that the transgenic potato plants exhibit a high potential in controlling CPB population and are a useful tool in the management of imidacloprid-resistant CPB.
dc.description.sponsorshipNigde Omer Halisdemir Scientific Research Unit (BAP Project) [FEB 2017/18 BAGEP]; TUBITAK [2215]
dc.description.sponsorshipThe present study was supported by Nigde Omer Halisdemir Scientific Research Unit (BAP Project No. FEB 2017/18 BAGEP). The authors are highly thankful to TUBITAK for providing PhD fellowship (code 2215) to Dr. Muhammad Salim to complete his doctoral studies. Authors are grateful to Prof. Dr. Mehmet Emin Calkan, Nigde Omer Halisdemir University and Mr. Cengiz Kurt, Trakya Agriculture Research Institute for providing seed tuber of potato and rice cultivar, respectively. Special thanks to Dr. Emre Aksoy for his technical help during experiments. We are also thankful to Prof. Dr. Sebahattin ozcan, University of Ankara and Prof. Dr. Josef Vlasak, Academy of Sciences of Czech Republic for providing recombinant plasmids of pTF101 and pET-22b(+), respectively.
dc.identifier.doi10.1007/s11816-021-00668-3
dc.identifier.endpage215
dc.identifier.issn1863-5466
dc.identifier.issn1863-5474
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85104253717
dc.identifier.scopusqualityQ2
dc.identifier.startpage197
dc.identifier.urihttps://doi.org/10.1007/s11816-021-00668-3
dc.identifier.urihttps://hdl.handle.net/11480/14288
dc.identifier.volume15
dc.identifier.wosWOS:000638831400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPlant Biotechnology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectGene stacking
dc.subjectPotato cultivars
dc.subjectInsect resistance
dc.subjectColorado potato beetle
dc.titleStacked insecticidal genes in potatoes exhibit enhanced toxicity against Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)
dc.typeArticle

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