EXPLORING THE AMENABILITY OF ONIONS TO AGROBACTERIUM MEDIATED TRANSFORMATION

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dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridGOKCE, ALI FUAT/0000-0002-2953-0326
dc.contributor.authorAmin, K.
dc.contributor.authorBakhsh, A.
dc.contributor.authorGokce, A. F.
dc.date.accessioned2024-11-07T13:24:07Z
dc.date.available2024-11-07T13:24:07Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe onions exhibit recalcitrant nature when subjected to genetic modifications. The study was conducted to optimize various factors affecting Agrobacterium mediated transformation in onion. Factors such as genotype dependency, type of explants and response of growth regulators were investigated. Agrobacterium strain LBA4404 harboring pBIN19 binary vector was used to infect different explants of two cultivars (Kral and Sampiyon). The T-DNA region of vector contained uidA gene (interrupted by an intronic sequence) to screen primary transgenic shoots at earlier stage and nptII gene as plant selectable marker. Results exhibited the genotype and explant dependency towards genetic modification of both cultivars. Based on the rate of callus induction, regeneration response and GUS histochemical assays, cultivar Kral was found better compared to Sampiyon. The best callus induction was observed on MS medium supplemented with 1: 10 ratio of 2,4-D and BAP respectively. Out of 355 primary transformants, 87 primary transformants were recorded as positive when subjected to PCR assays; 51 belonging to Kral showing tendency of genotype to genetic improvement. Results showed that highest number of putative transgenic plants was contributed by mature embryos followed by seeds and basal plates explants. Onion seed as explant has been used successfully for first time as no evidence in earlier literature was found. The optimization of these factors in Agrobacterium tumefaciens-mediated transformation will provide a gateway to introduce any desired trait in onion.
dc.description.sponsorshipTUBITAK; Scientific Research Projects Unit (BAP), Nigde Omer Halisdemir University [FEB 2016/18-YULTEP]
dc.description.sponsorshipThe authors acknowledge TUBITAK for providing fellowship to Ms. Khazina AMIN during her study towards MSc Thesis. The research was partially funded by Scientific Research Projects Unit (BAP), Nigde Omer Halisdemir University, under the Project No. FEB 2016/18-YULTEP. Seeds of onion cultivars were provided by MTN seed, Bandirma. We are thankful to Dr. Halil Toktay for providing access to microscope facilities.
dc.identifier.endpage771
dc.identifier.issn1018-7081
dc.identifier.issue3
dc.identifier.startpage761
dc.identifier.urihttps://hdl.handle.net/11480/13927
dc.identifier.volume29
dc.identifier.wosWOS:000467118500016
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherPakistan Agricultural Scientists Forum
dc.relation.ispartofJournal of Animal and Plant Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectGenetic transformation
dc.subjectonion
dc.subjectregeneration
dc.subjectGUS analysis
dc.subjecttransgenes
dc.titleEXPLORING THE AMENABILITY OF ONIONS TO AGROBACTERIUM MEDIATED TRANSFORMATION
dc.typeArticle

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