Investigation of Stem Cell Applications on In Vitro Fertilization in Rats

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dc.contributor.authorGumurdu, A.
dc.contributor.authorOzturk, S.
dc.contributor.authorAydemir, I
dc.contributor.authorTuglu, M., I
dc.date.accessioned2024-11-07T13:34:34Z
dc.date.available2024-11-07T13:34:34Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractWe aimed to search the effects of bone marrow-derived mesenchymal stem cell-conditioned media on in vitro fertilization by investigation of lifetime of germ cells cleavage, degeneration rates and embryo quality. For this purpose, firstly MSCs were isolated from femurs and tibias of the rat, and cells were cultured until the fourth passage. Sperm and oocytes were collected from male and female rats. Oocytes were added in Human Tuba! Fluid Media (HTFM), Single Step Media (SSM), Alpha-MEM Media (AMM) and Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Media (CM). Thousand sperm were added into the media which including oocytes. Embryos were allowed to produce by IVF. The development of the embryos vas followed until the 11th day, and the arrest, degeneration rates and alive embryos were established. The embryos readied 2. 4. 8. 16 cells stages and monda stage in the CM. While AMM had a negative effect on fertilization and embryo development. the most favourable effect was shown to be caused by CM in comparison with the other medias. These results have shorn that the beneficial effects of CM in IVF would be a significant increase in the rate of fertility and development of embryos.
dc.identifier.doi10.12681/jhvms.28489
dc.identifier.endpage3074
dc.identifier.issn1792-2720
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85119664175
dc.identifier.scopusqualityQ3
dc.identifier.startpage3067
dc.identifier.urihttps://doi.org/10.12681/jhvms.28489
dc.identifier.urihttps://hdl.handle.net/11480/16054
dc.identifier.volume72
dc.identifier.wosWOS:000713498000009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherHellenic Veterinary Medical Soc
dc.relation.ispartofJournal of the Hellenic Veterinary Medical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectBone Marrow Mesenchymal Stem Cell
dc.subjectIn Vitro Fertilization
dc.subjectOocyte
dc.subjectSperm
dc.subjectSuperovulation
dc.titleInvestigation of Stem Cell Applications on In Vitro Fertilization in Rats
dc.typeArticle

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