Clonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk

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dc.authoridAL, Serhat/0000-0003-2721-9275
dc.authoridERTAS ONMAZ, Nurhan/0000-0002-4679-6548
dc.authoridHIZLISOY, Harun/0000-0003-3391-0185
dc.authoridDinc, Gokcen/0000-0002-9184-5003
dc.contributor.authorHizlisoy, Harun
dc.contributor.authorOnmaz, Nurhan Ertas
dc.contributor.authorAl, Serhat
dc.contributor.authorKaradal, Fulden
dc.contributor.authorYildirim, Yeliz
dc.contributor.authorGonulalan, Zafer
dc.contributor.authorGumussoy, K. Semih
dc.date.accessioned2024-11-07T13:32:14Z
dc.date.available2024-11-07T13:32:14Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe aim of this study was the isolation, identification, phylogenetic analysis and antifungal susceptibility of Candida spp. from milk samples of healthy and mastitic cows in Kayseri/Turkey. Milk samples from 300 cows were found to be negative/positive for mastitis with the California Mastitis Test. Candida spp. was isolated by using the Brillance Candida Agar Base. Phenotypic tests, Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF), and VITEK-2 analyses were applied to confirm the obtained isolates. Resistance to flucytosine, fluconazole and caspofungin antifungals of the isolates were determined by Etest and VITEK-2. The genetic homologies of Candida spp. isolates were determined by Repetitive Extragenic Palindromic-PCR (RepPCR). In this study 62 (from 53 healthy, 9 mastitic) yeast isolates were obtained and 37 (59.6 %) were identified as non-albicans Candida (NAC) species. Phenotypic tests revealed that out of 62 isolates, 29 (46.7 %), 4 (6.5 %), 3 (4.8 %), 1 (1.6 %) and, 24 (38.7 %) were identified as Candida lusitaniae, Candida catenulate, Candida tropicalis, Candida silvicola and other yeast species, respectively. Only one sample (1.6 %) was identified as Candida albicans by MALDI-TOF however, according to VITEK-2, the agent was not confirmed as C. albicans. According to antifungal susceptibility testing by VITEK-2, one (2.7 %) of the isolates was resistant to fluconazole, one (2.7 %) was resistant to caspofungin, and 4 (10.8 %) were resistant to flucytosine. However, using E test, 10 isolates (27 %) were resistant to flucytosine. Using Rep-PCR, eight genotypic clones were observed. Genotype F (13.8 %) and G (13.8 %; 2 subtypes) were common clones in this study. In conclusion, NAC species were detected in healthy and mastitic cow milk samples. Epidemiological studies need to be conducted to track effectively the main source and to understand the diversity and distribution of the agent. It is necessary to consider the potential risks of yeast contamination in milk for public health. It is essential to focus on adequate sanitation procedures and storage conditions of milk.
dc.description.sponsorshipScientific Research Council of Erciyes University, Kayseri, Turkey [TCD-2015-5830]
dc.description.sponsorshipThis research was supported by the Scientific Research Council of Erciyes University, Kayseri, Turkey (Project No. TCD-2015-5830).
dc.identifier.doi10.15567/mljekarstvo.2020.0104
dc.identifier.endpage49
dc.identifier.issn0026-704X
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85078335039
dc.identifier.scopusqualityQ3
dc.identifier.startpage40
dc.identifier.urihttps://doi.org/10.15567/mljekarstvo.2020.0104
dc.identifier.urihttps://hdl.handle.net/11480/15307
dc.identifier.volume70
dc.identifier.wosWOS:000506215000004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCroatian Dairy Union
dc.relation.ispartofMljekarstvo
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectantifungal resistance
dc.subjectMALDI-TOF
dc.subjectVITEK-2
dc.subjectmolecular typing
dc.subjectmilk
dc.subjectnon-albicans Candida (NAC) species
dc.titleClonal diversity and antifungal susceptibility of Candida spp. recovered from cow milk
dc.title.alternativeKlonska raznolikost i antifungalna osjetljivost vrste Candida spp. izolirane iz mlijeka
dc.typeArticle

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