Expression of GNA and biting site-restricted cry1Ac in cotton; an efficient attribution to insect pest management strategies

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dc.contributor.authorKhabbazi S.D.
dc.contributor.authorKhabbazi A.D.
dc.contributor.authorÖzcan S.F.
dc.contributor.authorBakhsh A.
dc.contributor.authorBaşalma D.
dc.contributor.authorÖzcan S.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2018
dc.departmentNiğde ÖHÜ
dc.description.abstractInsect-resistant transgenic cotton has been commercialized for two decades. Most of the introduced cultivars express Bt gene(s) constitutively under the control of 35S promoter in whole-plant tissues. However, there have been other promoters considered by researchers to confine the toxin expression to targeted organ and tissues. We developed a triple-gene construct including GNA, cry1Ac and cp4 epsps genes. We attempted to confine cry1Ac expression to insect biting sites by cloning it to downstream of a wound-inducible promoter isolated from Asparagus officinalis (AoPR1). Moreover, to broaden the range of resistance, GNA was driven by the 35S promoter to target the sap-sucking insects like aphids which impose large losses in cotton production. To select the transformants in selection medium and for glyphosate tolerance, GNA and cry1Ac genes were accompanied with cp4 epsps gene. Two binary vectors harboring desired genes were constructed and utilized in the study (pGTGNAoC1AC and pGTGN35C1AC). Transformation of cultivar GSN-12 was carried out by employing Agrobacterium tumefaciens strain EHA105. Plantlets were primarily screened under glyphosate (N-phosphonomethyl glycine) selection pressure and subsequently subjected to molecular and biotoxicity assays. Introduction of cry1Ac and GNA to cotton plant conferred resistance to Spodoptera littoralis and Aphis gossypii Glover. Restriction of cry1Ac toxin protein to insect biting sites along with a plant lectin attributes significantly to insect pest management strategies. © 2018, Korean Society for Plant Biotechnology and Springer Japan KK, part of Springer Nature.
dc.description.sponsorshipAcknowledgements The PhD. fellowship awarded by The Scientific and Technological Research Council of Turkey (TUBITAK)-BIDEB to Dr. S.D. Khabbazi is deeply appreciated. The authors are grateful to the Leicester University (UK) for giving permission to use AoPR1 promoter for research purposes, Dr. Selma Onarıcı (TÜBİTAK GMBE) for providing pJIT61.cry1Ac plasmid and Prof. Umut Toprak (Department of Crop Protection, Ankara University) for providing S. littoralis larvae.
dc.identifier.doi10.1007/s11816-018-0493-8
dc.identifier.endpage282
dc.identifier.issn1863-5466
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85049581891
dc.identifier.scopusqualityQ2
dc.identifier.startpage273
dc.identifier.urihttps://dx.doi.org/10.1007/s11816-018-0493-8
dc.identifier.urihttps://hdl.handle.net/11480/1627
dc.identifier.volume12
dc.identifier.wosWOS:000439478500004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherSpringer Tokyo
dc.relation.ispartofPlant Biotechnology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAgglutinin lectin
dc.subjectBt
dc.subjectInsect pests
dc.subjectTransgenic cotton
dc.subjectWound-inducible promoter
dc.titleExpression of GNA and biting site-restricted cry1Ac in cotton; an efficient attribution to insect pest management strategies
dc.typeArticle

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