Heterologous expression of distinct insecticidal genes in potato cultivars encodes resistance against potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)

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dc.authoridSalim, Muhammad/0000-0003-3140-1299
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.contributor.authorSalim, Muhammad
dc.contributor.authorBakhsh, Allah
dc.contributor.authorNaqqash, Muhammad Nadir
dc.contributor.authorGokce, Ayhan
dc.date.accessioned2024-11-07T13:34:37Z
dc.date.available2024-11-07T13:34:37Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe potato tuber moth, Phthorimaea operculella (Zeller), is a notorious insect pest of potato incurring substantial yield losses in the field as well as in storage. Chemical control is difficult to exercise due to the latent feeding of the caterpillars and their ability to develop resistance against insecticides. One of the essential components of efficient insect-resistant management is using two or more different insecticidal genes in transgenic crops to effectively avoid and delay the resistance development in insect pests. Two constructs, namely DS-1 (cry3A + SN-19 genes) and DS-2 (OCII + SN-19 genes) in pCAMBIA1301 binary vector, were developed and were transformed in potato cultivars (Agria and Lady Olympia) via Agrobacterium-mediated transformation. The molecular analysis confirmed gene integration and expression of the introduced genes in transgenic plants. The insecticidal effects of incorporated genes in transgenic plants were assessed against 1st, 2nd, 3rd, and 4th instar potato tuber moth (PTM) larvae. The transgenic plants endured significantly high mortalities (100%) of larval stages of PTM within 72 h. Our results show that these transgenic potato plants have the potential to control populations of PTM and are also useful tools in managing PTM that would ultimately reduce the dependency on conventional chemical pesticides with potentially less or minimal hazards. These lines can also serve as an excellent source of germplasm for potato breeding program.
dc.description.sponsorshipNigde Omer Halisdemir Scientific Research Projects Unit [FEB 2017/18 BAGEP]
dc.description.sponsorshipThe authors are highly thankful to TUEBITAK for providing PhD fellowship (code 2215) to Dr. Muhammad Salim to complete his doctoral studies. The present study was supported by Nigde Omer Halisdemir Scientific Research Projects Unit (BAP Project No. FEB 2017/18 BAGEP).
dc.identifier.doi10.1007/s41348-024-00964-4
dc.identifier.endpage1505
dc.identifier.issn1861-3829
dc.identifier.issn1861-3837
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85198383606
dc.identifier.scopusqualityQ2
dc.identifier.startpage1493
dc.identifier.urihttps://doi.org/10.1007/s41348-024-00964-4
dc.identifier.urihttps://hdl.handle.net/11480/16057
dc.identifier.volume131
dc.identifier.wosWOS:001272201900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartofJournal of Plant Diseases and Protection
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectHeterologous expression
dc.subjectInsecticidal genes
dc.subjectPotato
dc.subjectProtection
dc.subjectPotato tuber moth
dc.titleHeterologous expression of distinct insecticidal genes in potato cultivars encodes resistance against potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)
dc.typeArticle

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