Melatonin modulates lipopolysaccharides-induced inflammatory response and maintains circadian rhythm associated with histone H3 acetylation in bovine mammary epithelial cells

Basit Öğe Kaydı
dc.authoridElsabagh, Mabrouk/0000-0002-0596-6547
dc.authoridKhanaki, Hassan/0000-0003-2645-7511
dc.authoridMa, Yi/0000-0002-3781-314X
dc.contributor.authorMa, Yi
dc.contributor.authorCheng, Long
dc.contributor.authorGao, Xingliang
dc.contributor.authorElsabagh, Mabrouk
dc.contributor.authorFeng, Yong
dc.contributor.authorLi, Zelin
dc.contributor.authorKhanaki, Hassan
dc.date.accessioned2024-11-07T13:35:03Z
dc.date.available2024-11-07T13:35:03Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractLipopolysaccharides (LPS) can disrupt the barrier function of mammary epithelial cells, leading to mastitis. The protective effects of melatonin (MT) have been demonstrated against various infections, but the underlying mechanism remains unclear in LPS-induced bovine mastitis. This study, therefore, aimed to investigate the modulatory effect of melatonin (MT) on LPS-induced inflammation in bovine mammary epithelial cells (BMECs), its influence on the disruption of circadian rhythms triggered by this inflammation, and the potential underlying molecular mechanisms. BMECs extracted from lactating cows underwent one of 4 treatments in triplicates for 12 h simultaneously: Control (CON), LPS (exposed to LPS at 1 mu g/mL), MT (exposed to MT at 40 mu mol/L), or LPS + MT (LMT, exposed to both LPS at 1 mu g/mL and MT at 40 mu mol/L). The flow cytometry results demonstrated that MT reduced the ratios of cells in the G0/G1 phase but increased those in the G2/M phase (P < 0.05). Additionally, MT inhibited p65 phosphorylation levels, leading to a decreased cellular inflammatory response (P < 0.05). Treatment with MT elevated the acetylation levels of histone H3 in BMECs compared to the LPS treatment (P < 0.05). The circadian rhythm analysis revealed a significant increase in the gene expression levels of BMAL1, PER1, CRY1, and CRY2 at the 12 h mark in LMT compared to the LPS treatment (P < 0.05). Additionally, this study assessed the fluctuations in clock-related genes PER2 and CLOCK over 24 h under various treatment conditions. The results demonstrated that BMECs exposed to LMT treatment exhibited a significant increase in the expression levels of CLOCK and PER2 genes from 4 to 24 h in comparison to the LPS treatment (P < 0.05). Moreover, cells in the LMT treatment displayed a more regular circadian rhythm cycle compared to those in the LPS treatment. Overall, the data suggest that MT could alleviate the inflammatory response in BMECs by upregulating histone H3 acetylation, thereby maintaining the physiological circadian rhythm of BMECs.
dc.description.sponsorshipNational key research and development program [2021YFC2103004]; Excellent Doctoral Dissertation Fund of Yangzhou University and International Academic Exchange Fund for Graduate Students of Yangzhou University; Construction and Demonstration of Ecological Circulation Safety Breeding Model for Meat Sheep in Southern Xinjiang [2021DB014]
dc.description.sponsorshipThis study was financially supported by the national key research and development program (2021YFC2103004) , Construction and Demonstration of Ecological Circulation Safety Breeding Model for Meat Sheep in Southern Xinjiang (2021DB014) , the Excellent Doctoral Dissertation Fund of Yangzhou University and International Academic Exchange Fund for Graduate Students of Yangzhou University. We thank the University of Melbourne and Xinjiang Academy of Agricultural and Reclamation Sciences for the cooperation that made this paper possible.
dc.identifier.doi10.1016/j.jff.2024.106156
dc.identifier.issn1756-4646
dc.identifier.issn2214-9414
dc.identifier.scopus2-s2.0-85189432531
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jff.2024.106156
dc.identifier.urihttps://hdl.handle.net/11480/16310
dc.identifier.volume116
dc.identifier.wosWOS:001222153500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Functional Foods
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectMelatonin
dc.subjectBovine mammary epithelial cells
dc.subjectHistone H3
dc.subjectInflammation
dc.subjectCircadian rhythm
dc.titleMelatonin modulates lipopolysaccharides-induced inflammatory response and maintains circadian rhythm associated with histone H3 acetylation in bovine mammary epithelial cells
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu