Effect of the temperature and mobile phase composition on the retention behavior of nitroanilines on ligand-exchange stationary phase

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dc.authorid0000-0002-4763-1374
dc.contributor.authorGurten, A. Ali
dc.contributor.authorUcan, Mustafa
dc.contributor.authorAbdullah, Meysun I.
dc.contributor.authorAyar, Ahmet
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2006
dc.departmentNiğde ÖHÜ
dc.description.abstractThis paper deals with the separation of isomers of nitroaniline by liquid chromatography using the ligand-exchange technique. The chromatographic separations were performed on the ligand-exchanger sporopollenin. The sporopollenin used as support of stationary phase was modified with carboxylated-ethylenediamine matrix and was loaded with cobalt(II) ions. Using the column packed with cobalt(II) loaded carboxylated diaminoethyl sporopollenin [Co(II)-CDA-E-S], the retention behavior of 3- and 4-nitroanilines was investigated. The mobile phase used, was a mixture of 0.05 M NH4OH in ethanol-water. The resolution was strongly affected by the presence of ammonium hydroxide in the mobile phase and a concentration of 0.05 M was shown to be necessary for the separation of analytes. To study the effects of temperature on the resolution. column runs were also performed at various temperatures (15-60 degrees C). With increasing temperature. a decreased interaction between the solutes and the ligand-exchanger was observed. Consequently. the best results were obtained using a mixture of 0.05M NH4OH in ethanol-water (10:90. v/v) as the mobile phase at a column temperature of 35 degrees C. Ligand-exchange chromatography on the Co(II)-CDAE-S could be a useful alternative method for the separation of nitroaniline. (c) 2005 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.jhazmat.2005.10.063
dc.identifier.endpage57
dc.identifier.issn0304-3894
dc.identifier.issue45352
dc.identifier.pmid16386366
dc.identifier.scopus2-s2.0-33646834594
dc.identifier.scopusqualityQ1
dc.identifier.startpage53
dc.identifier.urihttps://dx.doi.org/10.1016/j.jhazmat.2005.10.063
dc.identifier.urihttps://hdl.handle.net/11480/5498
dc.identifier.volume135
dc.identifier.wosWOS:000239133600008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofJOURNAL OF HAZARDOUS MATERIALS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectligand-exchange choromatography
dc.subjectsporopollenin
dc.subjectnitroaniline
dc.titleEffect of the temperature and mobile phase composition on the retention behavior of nitroanilines on ligand-exchange stationary phase
dc.typeArticle

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