Dietary rumen-protected L-arginine or N-carbamylglutamate attenuated fetal hepatic inflammation in undernourished ewes suffering from intrauterine growth restriction

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dc.authoridMa, Yi/0000-0002-3781-314X
dc.authoridElsabagh, Mabrouk/0000-0002-0596-6547
dc.authoridWang, Hongrong/0000-0002-4980-1082
dc.contributor.authorZhang, Hao
dc.contributor.authorZhang, Ying
dc.contributor.authorMa, Yi
dc.contributor.authorElsabagh, Mabrouk
dc.contributor.authorWang, Hongrong
dc.contributor.authorWang, Mengzhi
dc.date.accessioned2024-11-07T13:35:02Z
dc.date.available2024-11-07T13:35:02Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThis study aimed to explore whether dietary rumen-protected L-arginine (RP-Arg) or N-carbamylglutamate (NCG) supplementation to feed-restricted pregnant ewes counteracts fetal hepatic inflammation and innate immune dysfunction associated with intrauterine growth retardation (IUGR) in ovine fetuses. On d 35 of pregnancy, twin-bearing Hu ewes (n = 32) were randomly assigned to 4 treatment groups (8 ewes and 16 fetuses per group) and fed diets containing 100% of the NRC requirements (CON), 50% of the NRC requirements (RES), RES + RP-Arg (20 g/d) (RESA), or RES + NCG (5 g/d) (RESN). At 08:00 on d 110 of gestation, fetal blood and liver tissue samples were collected. The levels of triglyceride, free fatty acid, cholesterol and beta-hydroxybutyrate in the fetal blood of RESA and RESN groups were lower (P < 0.05) than those of the RES group, but were higher (P < 0.05) than those of the CON group. The interleukin (IL)6 and IL-1 levels in fetal blood and liver tissue as well as the myeloid differentiation primary response 88 (MyD88), transforming growth factor beta (TGF beta), and nuclear factor kappa B (NF-kB) mRNA levels in the fetal liver were decreased (P < 0.05) by the NCG or RP-Arg supplementation compared to the RES treatment. Similarly, the toll-like receptor (TLR)-4, MyD88, TGFb, and p-c-Jun N-terminal kinase (JNK) protein levels in the fetal liver were reduced (P < 0.05) in the NCG and RP-Arg-supplemented groups compared to the RES group. These results showed that dietary supplementation of RP-Arg or NCG to underfed pregnant ewes could protect against IUGR fetal hepatic inflammation via improving lipid metabolism, down-regulating the TLR-4 and the inflammatory JNK and NF-kB signaling pathways, and decreasing cytokine production in ovine fetal blood and liver tissue. (C) 2021 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.
dc.description.sponsorshipNational Natural Science Foundation of China [31902180]; Top Talents Award Plan of Yangzhou University; Cyanine Project of Yangzhou University; State Key Laboratory of Sheep Genetic Improvement and Healthy Production [2021ZD07]; Yangzhou University Science and Technology Innovation Foundation [2019CXJ152]
dc.description.sponsorshipThe research was supported by the fund for the National Natural Science Foundation of China (31902180), the Top Talents Award Plan of Yangzhou University (2019), the Cyanine Project of Yangzhou University (2020), the funds from State Key Laboratory of Sheep Genetic Improvement and Healthy Production (2021ZD07), and Yangzhou University Science and Technology Innovation Foundation (2019CXJ152). The authors thank all the members of Hong Rong Wang's laboratory who contributed to sample determination.
dc.identifier.doi10.1016/j.aninu.2021.06.004
dc.identifier.endpage1104
dc.identifier.issn2405-6383
dc.identifier.issn2405-6545
dc.identifier.issue4
dc.identifier.pmid34738040
dc.identifier.scopus2-s2.0-85122640114
dc.identifier.scopusqualityQ1
dc.identifier.startpage1095
dc.identifier.urihttps://doi.org/10.1016/j.aninu.2021.06.004
dc.identifier.urihttps://hdl.handle.net/11480/16301
dc.identifier.volume7
dc.identifier.wosWOS:000765999800021
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKeai Publishing Ltd
dc.relation.ispartofAnimal Nutrition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectL-arginine
dc.subjectFetal sheep
dc.subjectLiver inflammation
dc.subjectIntrauterine growth restriction
dc.subjectN-carbamylglutamate
dc.titleDietary rumen-protected L-arginine or N-carbamylglutamate attenuated fetal hepatic inflammation in undernourished ewes suffering from intrauterine growth restriction
dc.typeArticle

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