Determination of genotoxic effects of hookah smoking by micronucleus and chromosome aberration methods

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dc.contributor.authorEker E.D.
dc.contributor.authorKoyuncu H.
dc.contributor.authorŞahin N.Ö.
dc.contributor.authorYüksel A.
dc.contributor.authorBerköz M.
dc.contributor.authorDiler S.B.
dc.contributor.authorAkgül S.A.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractBackground: Use of a hookah (a type of water pipe) is a traditional way of smoking tobacco, particularly in the Middle East. In Turkey, its popularity has been growing in recent years, especially among young people. It is known that cigarette smoking has genotoxic effects and causes mutations, but no comprehensive study has been done on the genotoxic effects of hookah usage, particularly in Turkey. Material/Methods: We collected peripheral blood/buccal smear samples from 30 subjects who did not smoke cigarettes but who regularly smoke a hookah an average of 2 times per week, and from 30 control subjects who had never smoked cigarettes or a hookah. Chromosome analyses were performed on the samples obtained from peripheral blood of each individual, 25 metaphase plaques were counted for each, and chromosome/chromatid breakage/gap parameters were evaluated. Micronucleus analysis was done on buccal smear samples and micronucleus/binucleus parameters were investigated by counting 2000 cells of each individual. Results: Chromosome breakage ratios were found to be 0.64±0.86 and 0.46±0.71 in the study and control groups, respectively, while chromatid breakage ratios were 0.53±0.83 and 0.53±0.71; fragment ratios were 0.82±1.24 and 0.21±0.49 (p<0.05); and gap ratios were 0.57±0.83 and 0.18±0.53 (p<0.05), respectively. Micronucleus ratio was 6.03±2.06 and 4.43±2.27 (p<0.05) in the study and control groups, respectively, and binucleus ratios were 8.53±3.23 and 12.15±5.18, respectively (p<0.05). Conclusions: Results of our study reveal significant statistical differences between the individuals who smoked hookah and those who did not in terms of fragment, gap, micronucleus, and binucleus parameters, suggesting that smoking a hookah may cause genotoxic effects. © Med Sci Monit.
dc.identifier.doi10.12659/MSM.898593
dc.identifier.endpage4494
dc.identifier.issn1234-1010
dc.identifier.pmid27869111
dc.identifier.scopus2-s2.0-84997839790
dc.identifier.scopusqualityQ2
dc.identifier.startpage4490
dc.identifier.urihttps://dx.doi.org/10.12659/MSM.898593
dc.identifier.urihttps://hdl.handle.net/11480/1817
dc.identifier.volume22
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherInternational Scientific Literature Inc.
dc.relation.ispartofMedical Science Monitor
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectChromosome aberrations
dc.subjectMicronucleus tests
dc.subjectMutagenicity tests
dc.subjectSmoking
dc.titleDetermination of genotoxic effects of hookah smoking by micronucleus and chromosome aberration methods
dc.typeArticle

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