Identification of relationships among EDIL3, angiogenesis and inflammation in colorectal cancer: In-silico analysis
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bayrakol Medical Publisher
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim: The relationship between inflammation and the development of colorectal adenocarcinoma has been described for a number of years. In this cancer, chronic inflammation causes cellular damage and DNA mutations responsible for the proliferation of transformed cells. Epidermal growth factor-like repeats and discoidin I-like domains 3 (EDIL3/DEL-1) were anti-inflammatory protein, especially in epithelial cells. Our study, it was aimed to clarify the link of among IL-17A, EDIL3/DEL1, and the main angiogenic factor VEGFA/B in colorectal cancer. Materials and Methods: We investigated EDIL3, IL-17, and VEFGA/B mutations profile and levels of m-RNA expressions in 594 colorectal cancer patients using bioinformatics analysis for the revealing relationship among these molecules. Additionally, PolyPhen-2 and SNAP tools were used to predict and confirm the pathogenicity of the detected mutations. Results: In colorectal cancer patients, the IL-17 and EDIL3 expressions were inversely correlated but the low correlation coefficient was statistically significant. EDIL3 and VEGFB m-RNA levels were found to be statistically significantly lower in the patient group than in the healthy group. Furthermore, 10 mutations were identified in the EDIL3. The study predicted the potential pathological effect of eight mutations in the EDIL3. Discussion: Low expression of EDIL3 might contribute to anti-inflammation, supporting extravasation of leukocytes to inflammatory area by increased IL-17 expression. Although further studies to clarify the mechanisms explaining the role of EDIL3 in tumor angiogenesis in terms of VEGF gene, and the interaction between IL-17 and EDIL3 are required, the results indicate that EDIL3 may be a good novel molecular target gene in inflammatory pathways for colorectal cancer.
Açıklama
Anahtar Kelimeler
EDIL3/DEL-1, Colon cancer, Inflammation, Mutation, Gene expression, Angiogenesis
Kaynak
Annals of Clinical and Analytical Medicine
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
12
Sayı
3
