Comparative transcriptomics of drought stress response of taproot meristem region of contrasting purple carrot breeding lines supported by physio-biochemical parameters

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dc.authoridGOKCE, ALI FUAT/0000-0002-2953-0326
dc.authoridOZTURK, ZAHIDE NESLIHAN/0000-0002-2592-9997
dc.authoridJunaid, Muhammad Daniyal/0000-0003-0982-9264
dc.authoridChaudhry, Usman Khalid/0000-0002-5077-5141
dc.contributor.authorGokce, Zahide Neslihan Ozturk
dc.contributor.authorGokce, Ali Fuat
dc.contributor.authorJunaid, Muhammad Daniyal
dc.contributor.authorChaudhry, Usman Khalid
dc.date.accessioned2024-11-07T13:24:41Z
dc.date.available2024-11-07T13:24:41Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractCarrot is one of the nutritious vegetable crops sensitive to drought stress resulting in loss of quality and yield. There are a lot of studies on detailed molecular mechanisms of drought stress response of main crops; however, very little information available on vegetables, including carrots. Hence, in this study, we investigated root transcriptome profiles from the meristematic region of two contrasting purple carrot (B7262A, drought tolerant; P1129, drought sensitive) lines under varying stress levels (85% and 70%) by using RNA-Seq technique. The morpho-physiological and biochemical response of B7262A line exhibited tolerance behavior to both DS (85% and 70%). RNA-Seq analysis revealed that 15,839 genes were expressed commonly in both carrot lines. The carrot line B7262A showed regulation of 514 genes in response to 85% DS, whereas P1129 showed differential regulation of 622 genes under 70% DS. The B7262A carrot line showed higher upregulation of transcripts that suggested its resilient behavior contrary to P1129 line. Furthermore, validation of transcript gene by qRT-PCR also confirmed the RNA-Seq analysis resulting in elevated expression levels of MYB48 transcription factor, MAPK mitogen-activated protein kinase ANP1, GER geraniol 8-hydroxylase, ABA ABA-induced in somatic embryo 3, FBOX putative F-box protein, FRO ferric reduction oxidase, and PDR probable disease resistance protein. Current study provided unprecedented insights of purple carrot lines that can be potentially exploited for the screening and development of resilient carrot.
dc.description.sponsorshipTUBITAK-KAMAG [117G023]
dc.description.sponsorshipThis study was funded by TUBITAK-KAMAG with a project number of 117G023 (Kislik Sebze Islahinda Hat ve/veya Cesit Gelistirme).
dc.identifier.doi10.1007/s10142-022-00868-2
dc.identifier.endpage710
dc.identifier.issn1438-793X
dc.identifier.issn1438-7948
dc.identifier.issue4
dc.identifier.pmid35590117
dc.identifier.scopus2-s2.0-85130432703
dc.identifier.scopusqualityQ2
dc.identifier.startpage697
dc.identifier.urihttps://doi.org/10.1007/s10142-022-00868-2
dc.identifier.urihttps://hdl.handle.net/11480/14257
dc.identifier.volume22
dc.identifier.wosWOS:000798085200001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartofFunctional & Integrative Genomics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectAntioxidants
dc.subjectDrought stress
dc.subjectRNA-Seq
dc.subjectGene regulation
dc.subjectOsmoprotectants
dc.titleComparative transcriptomics of drought stress response of taproot meristem region of contrasting purple carrot breeding lines supported by physio-biochemical parameters
dc.typeArticle

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