Inhibiting effect of oleocanthal on neuroblastoma cancer cell proliferation in culture

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dc.authoridAYDEMIR, ISIL/0000-0002-4143-7319
dc.authoridunsal, ulkun unlu/0000-0001-5194-3138
dc.authoridTuglu, Mehmet Ibrahim/0000-0002-0569-8415
dc.contributor.authorUnsal, Ulkun Unlu
dc.contributor.authorMete, Mesut
dc.contributor.authorAydemir, Isil
dc.contributor.authorDuransoy, Yusuf Kurtulus
dc.contributor.authorUmur, Ahmet Sukru
dc.contributor.authorTuglu, Mehmet Ibrahim
dc.date.accessioned2024-11-07T13:34:26Z
dc.date.available2024-11-07T13:34:26Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractWe investigated the potential anticancer effects of oleocanthal (OC) on neuroblastoma cells. Cells were divided into four groups: group 1, neuroblastoma cells were treated with OC; group 2, neurons that differentiated from neuroblastoma cells were treated with phosphate-buffered saline(PBS); group 3, bone marrow derived neuronal (BMDN) cells that were differentiated from bone marrow derived mesenchymal stem cells (BMSCs) were treated with OC; group 4, BMDN cells that were differentiated from BMSCs were treated with PBS. Groups 2 and 4 were control groups. The effects of OC on cell viability, oxidative stress, neurite inhibition and apoptosis at IC50 dose were investigated using MTT analysis, i-NOS and e-NOS measurement, neurotoxicity screening test (NST) and TUNEL staining, respectively. MTT analysis demonstrated that cells were significantly less viable in group 1 than in group 3. i-NOS and e-NOS staining intensity was significantly greater in group 1 than in group 3. NST revealed that OC inhibited neurite growth in both neuroblastoma and BMND cells; inhibition was significantly less in group 3 than in group 1. Significantly more TUNEL labeled cells were found in group 1 than in group 3. We found that OC prevented growth and proliferation of neuroblastoma cells in culture by increasing oxidative stress and apoptosis. We also found that the cytotoxicity of OC is negligible in BMDN cells.
dc.identifier.doi10.1080/10520295.2019.1674919
dc.identifier.endpage241
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue3
dc.identifier.pmid31691588
dc.identifier.scopus2-s2.0-85074820678
dc.identifier.scopusqualityQ2
dc.identifier.startpage233
dc.identifier.urihttps://doi.org/10.1080/10520295.2019.1674919
dc.identifier.urihttps://hdl.handle.net/11480/15966
dc.identifier.volume95
dc.identifier.wosWOS:000494602100001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofBiotechnic & Histochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectapoptosis
dc.subjectcancer
dc.subjectneuroblastoma
dc.subjectoleocanthal
dc.subjectstem cells
dc.titleInhibiting effect of oleocanthal on neuroblastoma cancer cell proliferation in culture
dc.typeArticle

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