Detection and partial characterization of grapevine leafroll-associated virus 1 in pomegranate trees in Turkey

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dc.authorid0000-0002-6434-6321
dc.contributor.authorCaglayan, Kadriye
dc.contributor.authorElci, Eminur
dc.contributor.authorGazel, Mona
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractFoliar virus-like symptoms consisting of yellowing, chlorotic spots, oak-leaf and vein clearing were observed on pomegranate cultivar Hicaz in Hatay province of Turkey in 2013. Three symptomatic out of 23 pomegranate samples reacted to Grapevine leafroll-associated virus 1 (GLRaV-1) antibodies in DAS-ELISA. In order to confirm the presence of GLRaV-1 in pomegranate, total RNA extracted from petiole samples was used in RT-PCR using specific primers designed on sequences of the heat-shock protein 70 homolog (HSP70h), coat protein (CP), coat protein duplicate 2 (CPd2) and open reading frame 9 (p24) genes of GLRaV-1. Amplicons were only obtained from symptomatic pomegranate samples for the CP, CPd2, and p24 genes but, unlike for GLRaV-1 isolates from grapevine, no amplicon was obtained for the HSP70h gene of GLRaV-1 isolates from pomegranate. The CP, CPd2 and p24 genes of GLRaV-1 from pomegranate (accession no. KP411914-KP411922) had 91-94 % nucleotide sequence identity with GLRaV-1 isolates from grapevine. Phylogenetic analyses reconstructed using the neighbor joining method showed a clustering of GLRaV-1 isolates from pomegranate and grapevine. These results suggest that pomegranate could be an alternate host for GLRaV-1.
dc.identifier.doi10.1007/s10658-015-0807-4
dc.identifier.endpage202
dc.identifier.issn0929-1873
dc.identifier.issn1573-8469
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84946149234
dc.identifier.scopusqualityQ1
dc.identifier.startpage199
dc.identifier.urihttps://dx.doi.org/10.1007/s10658-015-0807-4
dc.identifier.urihttps://hdl.handle.net/11480/3659
dc.identifier.volume145
dc.identifier.wosWOS:000373994100018
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofEUROPEAN JOURNAL OF PLANT PATHOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPunica granatum L.
dc.subjectGLRaV-1
dc.subjectRT-PCR
dc.subjectSequencing
dc.titleDetection and partial characterization of grapevine leafroll-associated virus 1 in pomegranate trees in Turkey
dc.typeArticle

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