Transgenic potato lines expressing CP4-EPSP synthase exhibit resistance against glyphosate

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dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridDemirel, Ufuk/0000-0002-3457-5086
dc.contributor.authorBakhsh, Allah
dc.contributor.authorHussain, Tahira
dc.contributor.authorRahamkulov, Ilhom
dc.contributor.authorDemirel, Ufuk
dc.contributor.authorCaliskan, Mehmet Emin
dc.date.accessioned2024-11-07T13:24:48Z
dc.date.available2024-11-07T13:24:48Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPotato crops are particularly vulnerable to weed competition from emergence to canopy closure and are subject to significant yield loss. Glyphosate is broad spectrum herbicide used to control weeds worldwide. In order to incorporate glyphosate resistant trait in four potato cultivars (Lady Olympia, Desiree, Agria and Granola), an efficient, cost effective, reproducible and stable Agrobacterium-mediated genetic transformation protocol was performed using leaf and internodal explants. Agrobacterium strain LBA4404 harboring newly modified recombinant binary vector pCAMHE-EPSPS containing EPSP synthase gene under the control of Cauliflower mosaic virus 35S promoter was used to infect explants. The overall transformation efficiency was 26.4%. Of the 280 plants transferred to greenhouse, 74 plants were found to be PCR positive with gene of interest. GUS histochemical, Southern blot, RT-qPCR, lateral flow dipstick assays confirmed integration and expression of EPSPS in primary transformants. The putative transgenic plants developed from these cultivars possessed enhanced resistance to glyphosate applications in T0 and first tuber generation. These transgenic potato lines could be used as source of germplasm for an efficient potato breeding program. Key message The article reports the development of potato transgenic lines with enhanced tolerance against glyphosate.
dc.identifier.doi10.1007/s11240-019-01708-1
dc.identifier.endpage34
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85074565162
dc.identifier.scopusqualityQ1
dc.identifier.startpage23
dc.identifier.urihttps://doi.org/10.1007/s11240-019-01708-1
dc.identifier.urihttps://hdl.handle.net/11480/14333
dc.identifier.volume140
dc.identifier.wosWOS:000511716300003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPlant Cell Tissue and Organ Culture
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectPotato
dc.subjectGenetic manipulation
dc.subjectWeeds management
dc.subjectGlyphosate
dc.titleTransgenic potato lines expressing CP4-EPSP synthase exhibit resistance against glyphosate
dc.typeArticle

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