Functional analysis of Triticum durum type 1 metallothionein gene (dMT) in response to varying levels of cadmium

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dc.contributor.authorYesilirmak F.
dc.contributor.authorOzturk Gökçe Z.N.
dc.contributor.authorMetin B.
dc.contributor.authorSayers Z.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2018
dc.departmentNiğde ÖHÜ
dc.description.abstractThe effect of varying levels of cadmium and correlated changes on the expression level of a type 1 metallothionein gene (dMT) were investigated in Triticum durum cv. Balçalı-85. Increasing the cadmium concentration resulted in a decrease in the dry weights of roots and shoots, and the effect was stronger in roots. Roots also showed a higher capacity to accumulate cadmium. Southern blot analyses revealed that the dMT gene, delineated by two exons and a non-coding intron region, exists at a single locus in the T. durum genome. Changes in dMT gene expression during cadmium exposure were monitored by two approaches. Northern blot analyses showed that the transcript level in roots increased upon treatment with increasing cadmium, which was quantified by qRT-PCR as 4.5 fold of the base level at 10 µM Cd. These results show a positive correlation between cadmium exposure and expression of dMT gene in durum wheat, and will provide a basis for studies on the role of type 1 metallothioneins in cadmium response. © 2017, Indian Society for Plant Physiology.
dc.identifier.doi10.1007/s40502-017-0318-8
dc.identifier.endpage147
dc.identifier.issn0019-5502
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85045612248
dc.identifier.scopusqualityN/A
dc.identifier.startpage140
dc.identifier.urihttps://dx.doi.org/10.1007/s40502-017-0318-8
dc.identifier.urihttps://hdl.handle.net/11480/1685
dc.identifier.volume23
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherSpringer Verlag
dc.relation.ispartofIndian Journal of Plant Physiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCadmium
dc.subjectGene expression
dc.subjectMetallothionein
dc.subjectTriticum durum
dc.titleFunctional analysis of Triticum durum type 1 metallothionein gene (dMT) in response to varying levels of cadmium
dc.typeArticle

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