Combined Application of Biochar and Biocontrol Agents Enhances Plant Growth and Activates Resistance Against Meloidogyne incognita in Tomato

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dc.authoridAli, Muhammad Amjad/0000-0001-5381-7039
dc.authoridAzeem, Farrukh/0000-0002-2702-0330
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.contributor.authorArshad, Usman
dc.contributor.authorAzeem, Farrukh
dc.contributor.authorMustafa, Ghulam
dc.contributor.authorBakhsh, Allah
dc.contributor.authorToktay, Halil
dc.contributor.authorMcGiffen, Milt
dc.contributor.authorNawaz, Muhammad Amjad
dc.date.accessioned2024-11-07T13:35:26Z
dc.date.available2024-11-07T13:35:26Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBiochar (BC) is known to enhance plant growth and may activate plant resistance to various soil borne pathogens. In this experiment, the rice husk (RH) BC was combined with biocontrol agents (BCAs), Bacillus subtilis and Trichoderma harzianum against Meloidogyne incognita in tomato and the efficacy of BC and BCAs in boosting overall plant biomass assessed side by side. BCAs along with 3% RH BC were applied to potting soil, tomato plants were transplanted, and then the soil was inoculated with second stage juveniles of Meloidogyne incognita. Bacillus subtilis +3% RH BC improved overall plant growth and reduced M. incognita damage. To unravel the resistance at the molecular level, root samples were taken 6- and 12-days post inoculation (dpi) to measure the expression of two resistance genes, PR-1b and JERF3, with the help of quantitative real-time polymerase chain reaction (qRT-PCR). The expression of PR-1b was upregulated 6 dpi for only BCAs while JERF3 remained unchanged except in only Bacillus subtilis treated plants. Similarly, the expression of both genes was found to be upregulated in some combined treatments at 12 dpi. The results suggested that the 3% BC application with BCAs effectively manages root-knot nematode, enhances overall plant biomass, and triggers defense related genes in tomato plants.
dc.description.sponsorshipHigher Education Commission of Pakistan [9-5(Ph-1-MG-2)]; NRPU project [8097]
dc.description.sponsorshipThe authors are highly thankful to Higher Education Commission of Pakistan for supporting this research through Pak-Turk Researcher's Mobility Grant No. 9-5(Ph-1-MG-2) Pak-TurkR&D-HEC-2017 and NRPU project number 8097.
dc.identifier.doi10.1007/s10343-021-00580-4
dc.identifier.endpage601
dc.identifier.issn0367-4223
dc.identifier.issn1439-0345
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85113968397
dc.identifier.scopusqualityQ2
dc.identifier.startpage591
dc.identifier.urihttps://doi.org/10.1007/s10343-021-00580-4
dc.identifier.urihttps://hdl.handle.net/11480/16502
dc.identifier.volume73
dc.identifier.wosWOS:000691803500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofGesunde Pflanzen
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectBiochar
dc.subjectBiocontrol
dc.subjectMeloidogyne incognita
dc.subjectDefense-related genes
dc.subjectGene expression
dc.titleCombined Application of Biochar and Biocontrol Agents Enhances Plant Growth and Activates Resistance Against Meloidogyne incognita in Tomato
dc.title.alternativeDie kombinierte Anwendung von Biokohle und biologischen Schädlingsbekämpfungsmitteln verbessert das Pflanzenwachstum und aktiviert die Resistenz gegen Meloidogyne incognita bei Tomaten
dc.typeArticle

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