Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR

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dc.contributor.authorTaskin, Hatira
dc.contributor.authorBaktemur, Gokhan
dc.contributor.authorKurul, Mehmet
dc.contributor.authorBuyukalaca, Saadet
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2013
dc.departmentNiğde ÖHÜ
dc.description.abstractThis study was performed for comparison of meristem culture technique with shoot tip culture technique for obtaining virus-free plant, comparison of micropropagation success of two different nutrient media, and determination of effectiveness of real-time PCR assay for the detection of viruses. Two different garlic species (Allium sativum and Allium tuncelianum) and two different nutrient media were used in this experiment. Results showed that Medium 2 was more successful compared to Medium 1 for both A. tuncelianum and A. sativum (Kastamonu garlic clone). In vitro plants obtained via meristem and shoot tip cultures were tested for determination of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV) through real-time PCR assay. In garlic plants propagated via meristem culture, we could not detect any virus. OYDV and LYSV viruses were detected in plants obtained via shoot tip culture. OYDV virus was observed in amount of 80% and 73% of tested plants for A. tuncelianum and A. sativum, respectively. LYSV virus was found in amount of 67% of tested plants of A. tuncelianum and in amount of 87% of tested plants of A. sativum in this study.
dc.description.sponsorshipRepublic of Turkey Ministry of Food, Agriculture and Livestock-General Directorate of Agricultural Research and Policy (TAGEM) Research Projects Coordinating Office [TAGEM/11/AR-GE/20]; Roche Company; Cukurova University; Adana Veterinary Control Institute
dc.description.sponsorshipThe authors would like to thank the Republic of Turkey Ministry of Food, Agriculture and Livestock-General Directorate of Agricultural Research and Policy (TAGEM) Research Projects Coordinating Office (TAGEM/11/AR-GE/20), Roche Company, Cukurova University and Adana Veterinary Control Institute for supporting this research.
dc.identifier.doi10.1155/2013/781282
dc.identifier.issn1537-744X
dc.identifier.pmid23935432
dc.identifier.scopus2-s2.0-84880905014
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://dx.doi.org/10.1155/2013/781282
dc.identifier.urihttps://hdl.handle.net/11480/4501
dc.identifier.wosWOS:000321843500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherHINDAWI PUBLISHING CORPORATION
dc.relation.ispartofSCIENTIFIC WORLD JOURNAL
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleUse of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR
dc.typeArticle

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