Mendelian segregation pattern and expression studies of insecticidal gene (cry1Ac) in insect resistant cotton progeny

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dc.authorid0000-0003-3561-7863
dc.contributor.authorHussain, Tahira
dc.contributor.authorBakhsh, Allah
dc.contributor.authorMunir, Bushra
dc.contributor.authorHassan, Samina
dc.contributor.authorRao, Abdul Qayyum
dc.contributor.authorShahid, Ahmad Ali
dc.contributor.authorHusnain, Tayyab
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2014
dc.departmentNiğde ÖHÜ
dc.description.abstractThe progenies of transgenic lines Bt-14 and Bt-17 developed as an independent transformation event from a local cotton variety CIM-482 harboring two insecticidal genes (cry1Ac & cry2a) were evaluated to determine resistance against lepidopterans, mainly Helicoverpa armigera L. under field conditions. The standard molecular techniques, i.e. polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA) and western dot blot were used to confirm gene presence and expression level of transformed Bt gene, and its transfer pattern to further progeny. PCR confirmed integration of insecticidal gene in most of the plants in transgenic progeny, while expression of Bt gene quantified by ELISA and western dot blot showed variation in cry1Ac expression levels but interestingly, it conferred full protection against targeted insect pests. The leaf bioassays were conducted to determine the effectiveness of Bt genes against Helicoverpa armigera by calculating the mortality percentage of larvae. Most of the transgenic lines showed 70-100% mortality % age of Helicoverpa armigera. The agronomic characteristics of the transgenic lines were also recorded along with non transgenic control variety CIM-482. Morphological, agronomic and fibre data of these transgenic lines was recorded and analyzed statistically. Our results show that these transgenic lines (especially Bt-17 line) are promising cotton germplasm to be used in an efficient breeding programme.
dc.identifier.doi10.9755/ejfa.v26i8.17794
dc.identifier.endpage715
dc.identifier.issn2079-052X
dc.identifier.issn2079-0538
dc.identifier.issue8
dc.identifier.scopus2-s2.0-84902462676
dc.identifier.scopusqualityQ3
dc.identifier.startpage706
dc.identifier.urihttps://dx.doi.org/10.9755/ejfa.v26i8.17794
dc.identifier.urihttps://hdl.handle.net/11480/4143
dc.identifier.volume26
dc.identifier.wosWOS:000340858200006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherUNITED ARAB EMIRATES UNIV
dc.relation.ispartofEMIRATES JOURNAL OF FOOD AND AGRICULTURE
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCommercialization
dc.subjectGenetic approaches
dc.subjectResistant
dc.subjectTransformation
dc.titleMendelian segregation pattern and expression studies of insecticidal gene (cry1Ac) in insect resistant cotton progeny
dc.typeArticle

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