Calixarene-immobilized monolithic cryogels for preparative protein chromatography

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dc.contributor.authorGuven I.
dc.contributor.authorGezici O.
dc.contributor.authorBayrakci M.
dc.contributor.authorMorbidelli M.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2018
dc.departmentNiğde ÖHÜ
dc.description.abstractNew cation exchanger monolithic stationary phases were prepared by immobilization of three different calixarene derivatives (i.e. tetracarboxylate calix[4]arene, CLX-COO, tetrasulfonate calix[4]arene, CLX-SO3, and tetraphosphonate calix[4]arene, CLX-PO4) onto a monolithic cryogel support (i.e. poly(2-hydroksyethylmethacrilate-co-glycidyl methacrylate, P) and investigated with respect to preparative protein chromatography. The obtained monoliths were characterized through various techniques such as FTIR spectroscopy, isoelectric point measurements, titrimetric analyses, and mercury intrusion porosimetry. Protein retention was investigated using some model proteins (i.e. lysozyme, cytochrome c, and ?-chymotrypsinogen A, human serum albumin, and myoglobin), and the role of modifier (i.e. NaCl) concentration and pH was thoroughly analyzed under isocratic and gradient elution conditions. Overloading experiments were also conducted to study dynamic adsorption capacity and the obtained values were found to be ranging between 3 and 8 mg/mL depending on the type of calixarene molecule. Hence, higher or comparable protein adsorption capacities were seen to be applicable on calixarene-immobilized cryogels when compared to any other functionalized cryogels in the literature. Combined with the favorable properties of these monoliths, with respect to mass transport of large molecules, these results qualify calixarene functionalized monolithic cryogels as promising stationary phases for protein preparative purification. © 2018 Elsevier B.V.
dc.description.sponsorshipAuthors wish to thank TÜBİTAK (The Scientific and Technological Research Council of Turkey) for the financial support provided (Project number: 115Z236). Authors also wish to thank Nigde Ömer Halisdemir University, Karamanoglu Mehmetbey University, and ETH Zürich University for the facilities provided. Appendix A
dc.identifier.doi10.1016/j.chroma.2018.05.026
dc.identifier.endpage68
dc.identifier.issn0021-9673
dc.identifier.pmid29778445
dc.identifier.scopus2-s2.0-85046865653
dc.identifier.scopusqualityQ1
dc.identifier.startpage59
dc.identifier.urihttps://dx.doi.org/10.1016/j.chroma.2018.05.026
dc.identifier.urihttps://hdl.handle.net/11480/1630
dc.identifier.volume1558
dc.identifier.wosWOS:000436220900007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Chromatography A
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCalixarene
dc.subjectCation-exchange
dc.subjectCryogel
dc.subjectMonolithic column
dc.subjectSeparation
dc.subjectStationary phase
dc.titleCalixarene-immobilized monolithic cryogels for preparative protein chromatography
dc.typeArticle

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