Induction of liver fibrosis by CCl4 mediates pathological alterations in the spleen and lymph nodes: The potential therapeutic role of propolis

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dc.contributor.authorSayed, Eman A.
dc.contributor.authorBadr, Gamal
dc.contributor.authorHassan, Khadiga Abdel-Hameed
dc.contributor.authorWaly, Hanan
dc.contributor.authorOzdemir, Betul
dc.contributor.authorMahmoud, Mohamed H.
dc.contributor.authorAlamery, Salman
dc.date.accessioned2024-11-07T13:34:45Z
dc.date.available2024-11-07T13:34:45Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractIn an animal models, carbon tetrachloride (CCl4) is a carcinogenic agent that causes liver fibrosis. The current study aims to investigate whether induction in liver-fibrosis by CCl4 in the mouse model could promote the initiation of fibrosis in lymph node and spleen due to sustained increase of inflammatory signals and also aimed to clarify the protective therapeutic effects of propolis. The male mice (BALB/c) were categorized into three experimental sets and each group involved 15 mice. Control group falls into first group; group-II and group-III were injected with CCl4 to induce liver-fibrosis and oral supplementation with propolis was provided in group-III for 4-weeks. A major improvement with hepatic collagen and alpha-smooth muscle actin (alpha-SMA) production was aligned with the activation of liver fibrosis from CCl4. Mice treated with CCl4 exhibited collagen deposition towards liver sections, pathological alterations in spleen and lymph node architectures, and a significantly increase the circulation of both T&B cells in secondary lymphoid organs. Mechanically, the secondary lymphoid organs treated with CCl4 in mice exposed a positive growth in alpha-SMA and collagen expression, increased in proinflammatory cytokine levels and a significant increase in TGF-beta, NO and ROS levels. A manifest intensification in the expression of Nrf2, COX-2, and eNOS and upregulation of ASK1 and P38 phosphorylation. Interestingly, addition of propolis-treated CCl4 mice, substantially suppressed deposition of liver collagen, repealed inflammatory signals and resorted CCl4-mediated alterations in signaling cascades, thereby repairing the architectures of the secondary lymphoid organs. Our findings revealed benefits of propolis against fibrotic complications and enhancing secondary lymphoid organ architecture. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
dc.description.sponsorshipLaboratory of Immunology, Faculty of Science, Assiut University; King Saud University (Riyadh, Saudi Arabia) [RSP-2020/241]
dc.description.sponsorshipThe authors extend their appreciation to the Laboratory of Immunology, Faculty of Science, Assiut University, for supporting this research. Additionally, the authors would also like to extend their gratitude to the King Saud University (Riyadh, Saudi Arabia) for the funding of this research through Researchers Supporting Project number RSP-2020/241.
dc.identifier.doi10.1016/j.sjbs.2020.11.068
dc.identifier.endpage1282
dc.identifier.issn1319-562X
dc.identifier.issn2213-7106
dc.identifier.issue2
dc.identifier.pmid33613057
dc.identifier.scopus2-s2.0-85098214499
dc.identifier.scopusqualityQ1
dc.identifier.startpage1272
dc.identifier.urihttps://doi.org/10.1016/j.sjbs.2020.11.068
dc.identifier.urihttps://hdl.handle.net/11480/16154
dc.identifier.volume28
dc.identifier.wosWOS:000616723000004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofSaudi Journal of Biological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectCCl4
dc.subjectInflammation
dc.subjectLiver fibrosis
dc.subjectOxidative stress
dc.subjectPropolis
dc.subjectSecondary lymphoid organs
dc.titleInduction of liver fibrosis by CCl4 mediates pathological alterations in the spleen and lymph nodes: The potential therapeutic role of propolis
dc.typeArticle

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