Assessing genetic diversity of potato genotypes using inter-PBS retrotransposon marker system

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dc.authoridDemirel, Ufuk/0000-0002-3457-5086
dc.authoridBALOCH, Faheem Shehzad/0000-0002-7470-0080
dc.contributor.authorDemirel, Ufuk
dc.contributor.authorTindas, IlKnur
dc.contributor.authorYavuz, Caner
dc.contributor.authorBaloch, Faheem Shehzad
dc.contributor.authorCaliskan, Mehmet Emin
dc.date.accessioned2024-11-07T13:25:25Z
dc.date.available2024-11-07T13:25:25Z
dc.date.issued2018
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractHaving knowledge on genetic similarity and DNA profile of potato genotypes facilitates a breeder's decision for parent selection and provides accurate variety identification. Fingerprinting and identification of genetic similarity among 151 potato genotypes were achieved using an inter-primer-binding sites (iPBS) retrotransposon marker system. Our study is the first application of iPBS markers for fingerprinting and distinguishing large numbers of Solanum tuberosum genotypes. Initially, 16 potato genotypes were screened using 45 iPBS retrotransposon markers to identify polymorphisms. Seventeen of these primers were selected for fingerprinting the whole set of accessions due to strong, reproducible and polymorphic bands. The 17 iPBS primers produced 290 scorable bands of which 224 were polymorphic. The number of bands per primer ranged from 10 to 26 with an average of 17.1. The number of polymorphic bands per primer was between 6 and 21. The polymorphism percentage per primer ranged from 46.2 to 100.0% with an average of 77.2% per primer. The mean polymorphism information content (PIC) values of iPBS primers varied from 0.12 to 0.31 per primer. Genetic similarity based on Jaccard's coefficient of potato genotypes ranged from 0.61 to 0.93 with an average of 0.73. The data produced herein may be used for selection of appropriate parents and variety description in the future. The findings of the present study suggest that iPBS retrotransposons are powerful and easy DNA markers for fingerprinting the large samples of potato germplasm.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TuBTAK) [213O234]
dc.description.sponsorshipThis work was funded by Scientific and Technological Research Council of Turkey (TuBTAK), Project No. 213O234. Gratitude is extended to Prof. Dr Sedat Serce for excellent suggestions and scientific discussion on statistical analysis. We also thank Buse Leyla Cihangirolu for technical assistance during laboratory experiments. We greatly appreciate Dr Wayne Morris for his advice on improving the use of English in the manuscript.
dc.identifier.doi10.1017/S1479262117000041
dc.identifier.endpage145
dc.identifier.issn1479-2621
dc.identifier.issn1479-263X
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85013151862
dc.identifier.scopusqualityQ3
dc.identifier.startpage137
dc.identifier.urihttps://doi.org/10.1017/S1479262117000041
dc.identifier.urihttps://hdl.handle.net/11480/14690
dc.identifier.volume16
dc.identifier.wosWOS:000425490500005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCambridge Univ Press
dc.relation.ispartofPlant Genetic Resources-Characterization and Utilization
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectgenetic similarity
dc.subjectiPBS primers
dc.subjectmolecular characterization
dc.subjectpotato
dc.subjectTurkey
dc.titleAssessing genetic diversity of potato genotypes using inter-PBS retrotransposon marker system
dc.typeArticle

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