Investigation of vascular endothelial growth factor receptor-dependent neuroplasticity on rat nucleus tractus solitarius and phrenic nerve after chronic sustained hypoxia

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dc.authoridKULOGLU, NURHAN/0000-0002-1199-2784
dc.authoridBASARAN, KEMAL ERDEM/0000-0001-6035-9398
dc.contributor.authorKuloglu, Nurhan
dc.contributor.authorBasaran, Kemal E.
dc.contributor.authorYakan, Birkan
dc.date.accessioned2024-11-07T13:32:13Z
dc.date.available2024-11-07T13:32:13Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe neuronal system that controls respiration creates plasticity in response to physiological changes. Chronic sustained hypoxia causes neuroplasticity that contributes to ventilatory acclimatization to hypoxia (VAH). The purpose of this study is to explain the potential roles of the VAH mechanism developing because of chronic sustained hypoxia on respiratory neuroplasticity of vascular endothelial growth factor (VEGF) receptor activation on the nucleus tractus solitarius (NTS) and phrenic nerve. In this study 24 adult male Sprague-Dawley rats were used. Subjects were separated into four groups, a moderate-sham (mSHAM), severed-sham (sSHAM), moderate chronic sustained hypoxia (mCSH), and severed chronic sustained hypoxia (sCSH). Normoxic group (mSHAM and sSHAM) rats were exposed to 21% O-2 level (7 days) in the normobaric room while hypoxia group (mCSH and sCSH) rats were exposed to 13% and 10% O-2 level (7 days). Different protocols were applied for normoxic and hypoxia groups and ventilation, respiratory frequency, and tidal volume measurements were made with whole-body plethysmography. After the test HIF-1 alpha, erythropoietin (EPO), and VEGFR-2 expressions on the NTS region in the medulla oblongata and phrenic nerve motor neurons in spinal cord tissue were analyzed using the immunohistochemical stain method. Examinations on the medulla oblongata and spinal cord tissues revealed that HIF-1 alpha, EPO, and VEGFR-2 expressions increased in hypoxia groups compared to normoxic groups while a similar increase was also seen when respiratory parameters were assessed. Consequently, learning about VAH-related neuroplasticity mechanisms developed as a result of chronic continuous hypoxia will contribute to developing new therapeutical approaches to various diseases causing respiratory failure using brain plasticity without recourse to medicines.
dc.description.sponsorshipErciyes University the Scientific Research Projects Unit [TDK-2019-9121]
dc.description.sponsorshipThis study was supported by Erciyes University the Scientific Research Projects Unit, TDK-2019-9121 project code.
dc.identifier.doi10.1002/jbt.22918
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue12
dc.identifier.pmid34541741
dc.identifier.scopus2-s2.0-85115105887
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/jbt.22918
dc.identifier.urihttps://hdl.handle.net/11480/15285
dc.identifier.volume35
dc.identifier.wosWOS:000697906700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Biochemical and Molecular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectchronic sustained hypoxia
dc.subjectVEGFR-2
dc.subjectNTS
dc.subjectphrenic nerve
dc.titleInvestigation of vascular endothelial growth factor receptor-dependent neuroplasticity on rat nucleus tractus solitarius and phrenic nerve after chronic sustained hypoxia
dc.typeArticle

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