DEVELOPMENT OF A MICROPROPAGATION PROTOCOL FOR ENDANGERED HYPERICUM BILGEHAN-BILGILII BASKOSE & SAVRAN (HYPERICACEAE) SPECIES, LOCAL ENDEMIC TO TURKEY

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dc.contributor.authorTurker, Huseyin
dc.contributor.authorUnal, Bengu Turkyilmaz
dc.date.accessioned2024-11-07T13:25:07Z
dc.date.available2024-11-07T13:25:07Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractHypericum species, are medicinally important and well studied for their pharmaceutical values. These species are gradually losing ground due to habitat loss, serious anthropogenic reasons as well as excessive collection from nature. Hypericum bilgehan-bilgilii is a local endemic species in Turkey. The population size is small and facing a threat. Keeping this in view there was a need to develop an In vitro ex-situ conservation protocol. Therefore an attempt was made to develop a protocol for callus induction, shoot growth, rooting and acclimatization for this endemic species. The nodal parts of the plant were preferred as the source of explant. These were cultured on a MS medium containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and Kinetin (Kin) in different concentrations and combinations. The best callus induction was recorded in MS medium including 1.0 mg.L-1 Kin and 0.5 mg.L-1 2,4-D with 6.2 shoots per callus. MS media with different Kin concentrations were used for shoot growth. The best shoot growth was observed on the MS medium with 2.0 mg.L-1 Kin. Shoots grown In vitro were subcultured to MS medium containing 0 5 and 1.0 mg.L-1 indole-3-butyric acid (IBA) for rooting. The best-rooting frequency (90 %) and rooting were determined in MS medium containing 1.0 mg.L-1 IBA. There were 10 root formations per shoot. The micropropagated plantlets were acclimatized in pots filled with peat and by this way, an effective In vitro tissue culture protocol for the species studied was developed and its sustainable conservation way was confirmed.
dc.identifier.doi10.30848/PJB2022-3(44)
dc.identifier.endpage1095
dc.identifier.issn0556-3321
dc.identifier.issn2070-3368
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85130543448
dc.identifier.scopusqualityQ3
dc.identifier.startpage1089
dc.identifier.urihttps://doi.org/10.30848/PJB2022-3(44)
dc.identifier.urihttps://hdl.handle.net/11480/14526
dc.identifier.volume54
dc.identifier.wosWOS:000765037800036
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPakistan Botanical Soc
dc.relation.ispartofPakistan Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectHypericum bilgehan-bilgilii
dc.subjectEndemic
dc.subjectEx-situ conservation
dc.subjectCallus culture
dc.subjectMicropropagation
dc.titleDEVELOPMENT OF A MICROPROPAGATION PROTOCOL FOR ENDANGERED HYPERICUM BILGEHAN-BILGILII BASKOSE & SAVRAN (HYPERICACEAE) SPECIES, LOCAL ENDEMIC TO TURKEY
dc.typeArticle

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