Histopathological and epigenetic alterations in the spinal cord due to prenatal electromagnetic field exposure: An H3K27me3-related mechanism

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dc.authoridBiterge Sut, Burcu/0000-0001-5756-5756
dc.authoridIKINCI KELES, AYSE/0000-0003-0716-5695
dc.contributor.authorKeles, Ayse Ikinci
dc.contributor.authorSut, Burcu Biterge
dc.date.accessioned2024-11-07T13:25:15Z
dc.date.available2024-11-07T13:25:15Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractNeural system development is one of the most important stages of embryogenesis. Perturbations in this crucial process due to genetic and environmental risk factors cause neural tube defects and other central nervous system diseases. We investigated the effects of prenatal exposure to 900-MHz electromagnetic field (EMF) on the spinal cord. Pregnant rats were exposed to 900-MHz EMF for 1 h/day from E13.5 until birth. Six pups from the control and EMF groups were sacrificed at postnatal day 32, and the upper thoracic region of the spine was removed and processed for histological procedures. For histopathological analyses, hematoxylin&eosin staining and, for stereological analyses and the quantitation of motor neurons, cresyl violet staining was performed. H3K27me3 levels were determined via immunofluorescence staining. Histopathological analysis identified structural alterations of ependymal cells, enlarged central canals, as well as degenerated and shrunken motor neurons in the EMF group, while the control group tissues had normal appearances. We also observed enrichment of H3K27me3 in the ependymal cells and the motor neurons in the spinal cord of the control group rats, while the EMF group had low levels of H3K27me3 staining. Our results suggest that the loss of H3K27me3 signals might correlate with reduced neuronal stem cell potential in the EMF group and result in anatomical and structural differences in the spinal cord. This study provided a comprehensive histopathological analysis of the spinal cord after prenatal EMF exposure and offered an H3K27me3-dependent molecular explanation for the detrimental effects of EMF exposure on the spine.
dc.identifier.doi10.1177/0748233721996947
dc.identifier.endpage197
dc.identifier.issn0748-2337
dc.identifier.issn1477-0393
dc.identifier.issue4
dc.identifier.pmid33620299
dc.identifier.scopus2-s2.0-85101761026
dc.identifier.scopusqualityQ3
dc.identifier.startpage189
dc.identifier.urihttps://doi.org/10.1177/0748233721996947
dc.identifier.urihttps://hdl.handle.net/11480/14588
dc.identifier.volume37
dc.identifier.wosWOS:000627513800001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Inc
dc.relation.ispartofToxicology and Industrial Health
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectElectromagnetic field
dc.subjectspinal cord
dc.subjectstereology
dc.subjectependymal cells
dc.subjectmotor neuron
dc.titleHistopathological and epigenetic alterations in the spinal cord due to prenatal electromagnetic field exposure: An H3K27me3-related mechanism
dc.typeArticle

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