Mechanisms underlying the role of endoplasmic reticulum stress in the placental injury and fetal growth restriction in an ovine gestation model

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dc.contributor.authorZhang, Hao
dc.contributor.authorZha, Xia
dc.contributor.authorZheng, Yi
dc.contributor.authorLiu, Xiaoyun
dc.contributor.authorElsabagh, Mabrouk
dc.contributor.authorWang, Hongrong
dc.contributor.authorJiang, Honghua
dc.date.accessioned2024-11-07T13:35:04Z
dc.date.available2024-11-07T13:35:04Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground Exposure to bisphenol A (BPA), an environmental pollutant known for its endocrine-disrupting properties, during gestation has been reported to increase the risk of fetal growth restriction (FGR) in an ovine model of pregnancy. We hypothesized that the FGR results from the BPA-induced insufficiency and barrier dysfunction of the placenta, oxidative stress, inflammatory responses, autophagy and endoplasmic reticulum stress (ERS). However, precise mechanisms underlying the BPA-induced placental dysfunction, and subsequently, FGR, as well as the potential involvement of placental ERS in these complications, remain to be investigated. Methods In vivo experiment, 16 twin-pregnant (from d 40 to 130 of gestation) Hu ewes were randomly distributed into two groups (8 ewes each). One group served as a control and received corn oil once a day, whereas the other group received BPA (5 mg/kg/d as a subcutaneous injection). In vitro study, ovine trophoblast cells (OTCs) were exposed to 4 treatments, 6 replicates each. The OTCs were treated with 400 mu mol/L BPA, 400 mu mol/L BPA + 0.5 mu g/mL tunicamycin ( Tm; ERS activator), 400 mu mol/L BPA + 1 mu mol/L 4-phenyl butyric acid (4-PBA; ERS antagonist) and DMEM/ F12 complete medium (control), for 24 h. Results In vivo experiments, pregnant Hu ewes receiving the BPA from 40 to 130 days of pregnancy experienced a decrease in placental efficiency, progesterone (P4) level and fetal weight, and an increase in placental estrogen (E2) level, together with barrier dysfunctions, OS, inflammatory responses, autophagy and ERS in type A cotyledons. In vitro experiment, the OTCs exposed to BPA for 24 h showed an increase in the E2 level and related protein and gene expressions of autophagy, ERS, pro-apoptosis and inflammatory response, and a decrease in the P4 level and the related protein and gene expressions of antioxidant, anti-apoptosis and barrier function. Moreover, treating the OTCs with Tm aggravated BPA-induced dysfunction of barrier and endocrine (the increased E2 level and decreased P4 level), OS, inflammatory responses, autophagy, and ERS. However, treating the OTCs with 4-PBA reversed the counteracted effects of Tm mentioned above. Conclusions In general, the results reveal that BPA exposure can cause ERS in the ovine placenta and OTCs, and ERS induction might aggravate BPA-induced dysfunction of the placental barrier and endocrine, OS, inflammatory responses, and autophagy. These data offer novel mechanistic insights into whether ERS is involved in BPA-mediated placental dysfunction and fetal development.
dc.description.sponsorshipThe authors thank all the members of Hongrong Wang's laboratory for their contribution to sample determination. Additionally, we also thank Bullet Edits Limited for the linguistic editing and proofreading of the manuscript.
dc.description.sponsorshipThe authors thank all the members of Hongrong Wang's laboratory for their contribution to sample determination. Additionally, we also thank Bullet Edits Limited for the linguistic editing and proofreading of the manuscript.
dc.identifier.doi10.1186/s40104-023-00919-z
dc.identifier.issn1674-9782
dc.identifier.issn2049-1891
dc.identifier.issue1
dc.identifier.pmid37691111
dc.identifier.scopus2-s2.0-85170378982
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/s40104-023-00919-z
dc.identifier.urihttps://hdl.handle.net/11480/16318
dc.identifier.volume14
dc.identifier.wosWOS:001065063200001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBmc
dc.relation.ispartofJournal of Animal Science and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectAutophagy
dc.subjectBisphenol A
dc.subjectEndoplasmic reticulum stress
dc.subjectFetal growth restriction
dc.subjectInflammatory responses
dc.subjectSheep
dc.titleMechanisms underlying the role of endoplasmic reticulum stress in the placental injury and fetal growth restriction in an ovine gestation model
dc.typeArticle

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