AN EFFICIENT AND COST EFFECTIVE STERILIZING METHOD WITH LEAST MICROBIAL CONTAMINATION AND MAXIMUM GERMINATION RATIO FOR IN VITRO COTTON (Gossypium hirsutum L.) CULTURE
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
PAKISTAN AGRICULTURAL SCIENTISTS FORUM
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cotton (Gossypium hirsutum L.) is a highly significant crop because of its quality for textile industry of the world. With the advent of genetic engineering, cotton has gone through several genetic improvements that encode tolerance against biotic and abiotic stresses. The efficient seed sterilization method is essential for successful genetic improvement in cotton. The present study was conducted to compare different sterilizing agents employed for in vitro culture of cotton to find out an efficient, reproducible, cost effective and genotype independent in vitro seed sterilization procedure of cotton. The two cultivated cotton cultivars i.e. GSN-12 and Coker-312 were used for these studies that were further transformed with insecticidal genes under different promoters. The different sterilizing agents used (in separate or combination) were ethanol, commercial bleach (NaOCl), mercuric chloride (HgCl2), Sodium Dodecyle (SDS), hydrogen peroxide (H2O2). The surface sterilized seeds of both cultivars were cultured on MS medium and data was recorded for contamination free seeds and germination ratio. The results showed that 5% n-Hexane in combination with 2% of H2O2 are the best sterilizing agents for both cultivars when compared to the efficacy of other chemicals. Seeds treated with n-hexane and hydrogen peroxide had minimum microbial contamination with improved germination rate. The sterilizing method was successfully used for surface sterilization of other cotton cultivars in our project. According to our knowledge, this is the first report of using n-hexane in combination with H2O2 for surface sterilization of cotton seed for in vitro culture studies.
Açıklama
Anahtar Kelimeler
microbe contamination, cotton sterilization, germination, efficiency, tissue culture
Kaynak
JOURNAL OF ANIMAL AND PLANT SCIENCES
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
26
Sayı
3
