Novel humic acid-bonded magnetite nanoparticles for protein immobilization

Basit Öğe Kaydı
dc.authorid0000-0001-6808-2282
dc.contributor.authorBayrakci, Mevlut
dc.contributor.authorGezici, Orhan
dc.contributor.authorBas, San Zeki
dc.contributor.authorOzmen, Mustafa
dc.contributor.authorMaltas, Esra
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2014
dc.departmentNiğde ÖHÜ
dc.description.abstractThe present paper is the first report that introduces (i) a useful methodology for chemical immobilization of humic acid (HA) to aminopropyltriethoxysilane-functionalized magnetite iron oxide nanoparticles (APS-MNPs) and (ii) human serum albumin (HSA) binding to the obtained material (HA-APS-MNPs). The newly prepared magnetite nanoparticle was characterized by using Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and elemental analysis. Results indicated that surface modification of the bare magnetite nanoparticles (MNPs) with aminopropyltriethoxysilane (APS) and HA was successfully performed. The protein binding studies that were evaluated in batch mode exhibited that HA-APS-MNPs could be efficiently used as a substrate for the binding of HSA from aqueous solutions. Usually, recovery values higher than 90% were found to be feasible by HA-APS-MNPs, while that value was around 2% and 70% in the cases of MNPs and APS-MNPs, respectively. Hence, the capacity of MNPs was found to be significantly improved by immobilization of HA. Furthermore, thermal degradation of HA-APS-MNPs and HSA bonded HA-APS-MNPs was evaluated in terms of the Horowitz-Metzger equation in order to determine kinetic parameters for thermal decomposition. Activation energies calculated for HA-APS-MNPs (20.74 kJ mol(-1)) and HSA bonded HA-APS-MNPs (33.42 kJ mol(-1)) implied chemical immobilization of HA to APS-MNPs, and tight interactions between HA and HA-APS-MNPs. (C) 2014 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK through the 2219-International Post-Doctoral Research Fellowship Programme
dc.description.sponsorshipThe authors wish to thank Nigde University and Selcuk University for the facilities provided. One of the authors (O. Gezici) wishes to thank TUBITAK for the scholarship provided through the 2219-International Post-Doctoral Research Fellowship Programme, and ETH Zurich for library facilities.
dc.identifier.doi10.1016/j.msec.2014.05.066
dc.identifier.endpage552
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.pmid25063152
dc.identifier.scopus2-s2.0-84903139028
dc.identifier.scopusqualityQ1
dc.identifier.startpage546
dc.identifier.urihttps://dx.doi.org/10.1016/j.msec.2014.05.066
dc.identifier.urihttps://hdl.handle.net/11480/4135
dc.identifier.volume42
dc.identifier.wosWOS:000340687400069
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHumic substances
dc.subjectHuman serum albumin (HSA)
dc.subjectMagnetite nanoparticle
dc.subjectProtein binding
dc.subjectHorowitz-Metzger method
dc.titleNovel humic acid-bonded magnetite nanoparticles for protein immobilization
dc.typeArticle

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