Thymoquinone improves testicular damage and sperm quality in experimentally varicocele-induced adolescent rats

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dc.authoridSenturk, Meryem/0000-0002-3763-8310
dc.authoridGUR, FATIH MEHMET/0000-0001-7748-3272
dc.authoridNurdinov, Nursultan/0000-0001-5341-7211
dc.contributor.authorGur, Fatih Mehmet
dc.contributor.authorTimurkaan, Sema
dc.contributor.authorTaskin, Eylem
dc.contributor.authorGuven, Celal
dc.contributor.authorGur, Hatice Emel
dc.contributor.authorSenturk, Meryem
dc.contributor.authorDastan, Sevgi
dc.date.accessioned2024-11-07T13:34:53Z
dc.date.available2024-11-07T13:34:53Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe aim of this study was to investigate the protective and therapeutic effects of thymoquinone against the negative effects of varicocele on testicular tissue and sperm morphology. Five groups were formed by random selection from a total of 40 adult male Wistar rats (n = 8). Thymoquinone (5 mg/kg/day) was administered intraperitoneally to the varicocele-dimethyl sulfoxide-olive oil-thymoquinone (VT) group and the sham-thymoquinone group. At the end of the 60th day, all groups were anaesthetised and the left testis was removed from the body quickly. One half of the testis tissue, which was divided into two, was separated for biochemical and Western blot analysis, while the other half were fixed in Bouin's fixative. As a result of biochemical, molecular and histopathological analyses, a statistically significant increase was found in the varicocele group testicular tissues in the malondialdehyde level, apoptotic index, Bax expression, cytochrome c expression and Bax/Bcl-2 ratio compared with the sham group. In addition, histopathological changes characterised by partial or complete degeneration of the germinal epithelium were observed in the seminiferous tubules in the same group. Total oxidant status level and sperm count with abnormal morphology increased in varicocele group, whereas total antioxidant status level decreased. In the VT group, all of the biochemical, molecular and histopathological changes detected in the varicocele group were statistically significantly reduced. When the findings obtained in this study are evaluated, it can be said that thymoquinone has the potential to be used as a preventive and therapeutic pharmacological agent in the medical treatment of varicocele. Although the exact mechanism of action of thymoquinone has not been fully elucidated, the findings obtained in this study support the view that thymoquinone showed a cytoprotective effect by reducing apoptosis, oxidative stress and lipid peroxidation.
dc.description.sponsorshipScientific Research Projects Unit of Nigde Omer Halisdemir Universityr [SSB 2018/02-BAGEP]
dc.description.sponsorshipThis work was supported by Scientific Research Projects Unit of Nigde Omer Halisdemir Universityr, Grant/Award Number: SSB 2018/02-BAGEP
dc.identifier.doi10.1111/and.14033
dc.identifier.issn0303-4569
dc.identifier.issn1439-0272
dc.identifier.issue5
dc.identifier.pmid33660882
dc.identifier.scopus2-s2.0-85101913896
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1111/and.14033
dc.identifier.urihttps://hdl.handle.net/11480/16224
dc.identifier.volume53
dc.identifier.wosWOS:000625166700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofAndrologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectBax/Bcl-2
dc.subjectcytochrome c
dc.subjecttestis
dc.subjectthymoquinone
dc.subjectvaricocele
dc.titleThymoquinone improves testicular damage and sperm quality in experimentally varicocele-induced adolescent rats
dc.typeArticle

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