Exploring Wild and Local Fruits as Sources of Promising Biocontrol Agents against Alternaria spp. in Apples
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mdpi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Biological control agents (BCAs) are a promising option for managing postharvest diseases. Their environmentally friendly nature makes them valuable for sustainable and eco-friendly postharvest disease management. This study evaluated the antagonistic potential of epiphytic yeasts isolated from a local apple genotype known as Nigde Elmasi and a range of wild fruits: rosehip, hawthorn, and wild pear. There were 375 yeast isolates obtained and screened in vitro and in vivo. Initially selected were 32 isolates able to inhibit the growth of Alternaria alternata mycelia in the in vitro experiments and identified using molecular methods as candidate BCAs. These isolates were identified as Aureobasidium pullulans using sequence analysis of the internal transcribed spacer (ITS) and the translation elongation factor EF-1 alpha gene (EF1 alpha). Based on two rounds of in vivo screening, four isolates were subsequently selected for their capability to control the infection of apple fruits under high inoculum pressure. Finally, their volatile and non-volatile antimicrobial activity was tested against Alternaria spp. These findings showed how wild fruits and a local apple genotype could represent a promising source for new BCA isolation. However, further studies are needed to reveal the mechanisms of action of these putative BCAs for application during the postharvest processing and storage of apples.
Açıklama
Anahtar Kelimeler
Aureobasidium spp., antagonist, black rot, rosehip, hawthorn, wild pear
Kaynak
Horticulturae
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
9
Sayı
10
