In vivo cartilage tissue engineering

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dc.contributor.authorGurer, B.
dc.contributor.authorCabuk, S.
dc.contributor.authorKarakus, O.
dc.contributor.authorYilmaz, N.
dc.contributor.authorYilmaz, C.
dc.date.accessioned2024-11-07T10:40:20Z
dc.date.available2024-11-07T10:40:20Z
dc.date.issued2018
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground: Biologic treatment options for cartilage injuries require chondrocyte expansion using cell culture. Clinical application is accomplished in two surgical sessions and is expensive. If isolation of chondrocytes and stimulus for proliferation and extracellular matrix synthesis can be achieved in vivo, the treatment can be performed in one session and the cost can be reduced. Methods: A 2.5-cm diameter full-thickness chondral defect was created in the knees of five groups of sheep. In one group, some of the chondral tissues obtained from the creation of the defect were diced into small pieces and were placed into the defect and were covered with a collagen membrane (MIV group). In the other group, the collagen membrane was soaked in collagenase prior to usage. In the next group, the collagen membrane was soaked in both collagenase and growth factors. Matrix-induced autologous chondrocyte implantation (MACI) was applied to another group in two sessions, and the last group was left untreated. After 15 weeks of follow-up, repair tissues were compared macroscopically, histomorphometrically, and biochemically for tissue concentrations of glycosaminoglycan and type II collagen. Results: MACI and MIV groups demonstrated better healing than others and were similar. Addition of collagenase or growth factors did not improve the results. Addition of collagenase did not have detrimental effect on the surrounding cartilage. Conclusions: With the described method, it is possible to obtain comparable results with MACI. Further studies are also needed to see if it works similarly in humans. © 2018 The Author(s).
dc.identifier.doi10.1186/s13018-018-0823-0
dc.identifier.issn1749-799X
dc.identifier.issue1
dc.identifier.pmid29739464
dc.identifier.scopus2-s2.0-85046628834
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/s13018-018-0823-0
dc.identifier.urihttps://hdl.handle.net/11480/11599
dc.identifier.volume13
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.relation.ispartofJournal of Orthopaedic Surgery and Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectCartilage diseases
dc.subjectCatilage matrix protein
dc.subjectCollagen-induced
dc.subjectKnee injuries
dc.titleIn vivo cartilage tissue engineering
dc.typeArticle

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