Chromosome-specific barcode system with centromeric repeat in cultivated soybean and wild progenitor

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dc.contributor.authorTek, Ahmet L.
dc.contributor.authorNagaki, Kiyotaka
dc.contributor.authorYıldız Akkamış, Hümeyra
dc.contributor.authorTanaka, Keisuke
dc.contributor.authorKobayashi, Hisato
dc.date.accessioned2024-11-07T10:40:14Z
dc.date.available2024-11-07T10:40:14Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractWild soybean Glycine soja is the progenitor of cultivated soybean Glycine max. Information on soybean functional centromeres is limited despite extensive genome analysis. These species are an ideal model for studying centromere dynamics for domestication and breeding. We performed a detailed chromatin immunopre-cipitation analysis using centromere-specific histone H3 protein to delineate two distinct centromeric DNA sequences with un-usual repeating units with monomer sizes of 90–92 bp (CentGm-1) and 413-bp (CentGm-4) shorter and longer than standard nu-cleosomes. These two unrelated DNA sequences with no sequence similarity are part of functional centromeres in both species. Our results provide a comparison of centromere properties between a cultivated and a wild species under the effect of the same kinetochore protein. Possible sequence homogenization specific to each chromosome could highlight the mechanism for evolutionary conservation of centromeric properties independent of domestication and breeding. Moreover, a unique barcode system to track each chromosome is developed using CentGm-4 units. Our results with a unifying centromere composition model using CentGm-1 and CentGm-4 superfamilies could have far-reaching implications for comparative and evolutionary genome research. © 2024 Tek et al.
dc.description.sponsorshipJapan Society for the Promotion of Science, JSPS; Tokyo University of Agriculture, TUA; Okayama University; Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (1210014, 121O014)
dc.identifier.doi10.26508/lsa.202402802
dc.identifier.issn2575-1077
dc.identifier.issue12
dc.identifier.pmid39353738
dc.identifier.scopus2-s2.0-85205526859
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.26508/lsa.202402802
dc.identifier.urihttps://hdl.handle.net/11480/11530
dc.identifier.volume7
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherLife Science Alliance, LLC
dc.relation.ispartofLife Science Alliance
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectCentromere
dc.subjectChromosomes, Plant
dc.subjectDNA Barcoding, Taxonomic
dc.subjectDNA, Plant
dc.subjectDomestication
dc.subjectGenome, Plant
dc.subjectGlycine max
dc.subjectHistones
dc.subjectPlant Breeding
dc.subjecthistone
dc.subjectplant DNA
dc.subjectcentromere
dc.subjectDNA barcoding
dc.subjectdomestication
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectplant breeding
dc.subjectplant chromosome
dc.subjectplant genome
dc.subjectprocedures
dc.subjectsoybean
dc.titleChromosome-specific barcode system with centromeric repeat in cultivated soybean and wild progenitor
dc.typeArticle

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