The effects of pollen, propolis, and caffeic acid phenethyl ester on tyrosine hydroxylase activity and total RNA levels in hypertensive rats caused by nitric oxide synthase inhibition: experimental, docking and molecular dynamic studies

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dc.authoridDurdagi, Serdar/0000-0002-0426-0905
dc.authoridEkhteiari Salmas, Ramin/0000-0003-3888-5070
dc.authoridSelamoglu, Zeliha/0000-0001-9056-6435
dc.contributor.authorSalmas, Ramin Ekhteiari
dc.contributor.authorDurdagi, Serdar
dc.contributor.authorGulhan, Mehmet Fuat
dc.contributor.authorDuruyurek, Merve
dc.contributor.authorAbdullah, Huda I.
dc.contributor.authorSelamoglu, Zeliha
dc.date.accessioned2024-11-07T13:34:56Z
dc.date.available2024-11-07T13:34:56Z
dc.date.issued2018
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe objective of the present study was to evaluate the effects of propolis, pollen, and caffeic acid phenethyl ester (CAPE) on tyrosine hydroxylase (TH) activity and total RNA levels of N-nitro-L-arginine methyl ester (L-NAME) inhibition of nitric oxide synthase in the heart, adrenal medulla, and hypothalamus of hypertensive male Sprague dawley rats. The TH activity in the adrenal medulla, heart, and hypothalamus of the rats was significantly increased in the L-NAME group vs. control (p<0.05). Treatment with L-NAME led to a significant increase in blood pressure (BP) in the L-NAME group compared to control (p<0.05). These data suggest that propolis, pollen, and CAPE may mediate diminished TH activity in the heart, adrenal medulla, and hypothalamus in hypertensive rats. The decreased TH activity may be due to the modulation and synthesis of catecholamines and BP effects. In addition, the binding mechanism of CAPE within the catalytic domain of TH was investigated by means of molecular modeling approaches. These data suggest that the amino acid residues, Glu429 and Ser354 of TH may play a pivotal role in the stabilization of CAPE within the active site as evaluated by molecular dynamics (MD) simulations. Gibbs binding free energy (G(binding)) of CAPE in complex with TH was also determined by post-processing MD analysis approaches (i.e. Poisson-Boltzmann Surface Area (MM-PBSA) method).
dc.identifier.doi10.1080/07391102.2017.1288660
dc.identifier.endpage620
dc.identifier.issn0739-1102
dc.identifier.issn1538-0254
dc.identifier.issue3
dc.identifier.pmid28132600
dc.identifier.scopus2-s2.0-85012879564
dc.identifier.scopusqualityQ2
dc.identifier.startpage609
dc.identifier.urihttps://doi.org/10.1080/07391102.2017.1288660
dc.identifier.urihttps://hdl.handle.net/11480/16252
dc.identifier.volume36
dc.identifier.wosWOS:000424485200005
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofJournal of Biomolecular Structure & Dynamics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjecthypertension
dc.subjectL-NAME
dc.subjecttyrosine hydroxylase
dc.subjectpollen
dc.subjectpropolis
dc.subjectCAPE
dc.subjectmolecular dynamics (MD) simulations
dc.subjectMM-PBSA
dc.titleThe effects of pollen, propolis, and caffeic acid phenethyl ester on tyrosine hydroxylase activity and total RNA levels in hypertensive rats caused by nitric oxide synthase inhibition: experimental, docking and molecular dynamic studies
dc.typeArticle

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