Establishment of highly efficient and reproducible Agrobacterium-mediated transformation system for tomato (Solanum lycopersicum L.)

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dc.authoridHashmi, Muneeb Hassan/0000-0002-2873-5661
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridDemirel, Ufuk/0000-0002-3457-5086
dc.contributor.authorHashmi, Muneeb Hassan
dc.contributor.authorSaeed, Faisal
dc.contributor.authorDemirel, Ufuk
dc.contributor.authorBakhsh, Allah
dc.date.accessioned2024-11-07T13:25:29Z
dc.date.available2024-11-07T13:25:29Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractA simple and improved Agrobacterium-mediated transformation protocol of tomato (Solanum lycopersicum) cultivar Rio Grande was developed to inspect the potential of producing transgenic tomatoes. In this study, regeneration and transformation efficiency as assessed in response to the seedling age, explant type, co-infection, co-cultivation duration, selection pressure (kanamycin), and the optimal concentration of plant growth regulators (PGR) 6-benzylaminopurine (BAP), gibberellic acid (GA3), and indolebutyric acid (IBA) in a Murashige and Skoog (MS) basal medium. To accomplish this goal, 2- to 4-wk-old tomato explant cotyledons, hypocotyl, and cotyledonary nodes were excised and transformed with the EHA105 Agrobacterium tumefaciens strain harboring pBIN19 binary vector containing uidA reporter gene and nptII as a selectable marker. Results revealed that 14-d-old cotyledonary nodes and leaves inoculated for 15 min with A. tumefaciens strain EHA105 following 48-h co-cultivation were optimal for the highest percent transformation efficiency 27.31. Antibiotic kanamycin (Kan) at 25 mg L-1 in the regeneration selection medium was found to be effective. MS medium containing optimal concentrations of 1.5 mg L-1 of BAP, 0.2 mg L-1 GA(3), and IBA 1.5 mg L-1 showed a significant level of percent regeneration, shoot elongation, and rooting efficiency of transformed plantlets. Molecular analysis of T-0 transgenic tomato plants showed integration and a higher relative expression level of the uidA gene. The optimized A. tumefaciens-mediated transformation method for tomato cultivar Rio Grande showed the highest percent transformation efficiency (TE) and regeneration efficiency (RE) and is likely to give consistent results with different tomato cultivars.
dc.description.sponsorshipScientific Research projects Unit (BAP) of Nigde Omer Halisdemir University (NOHU), Nigde, Turkey [TGT 2020/17-LUTEP]; Ayhan Sahenk Foundation
dc.description.sponsorshipWe received from the Scientific Research projects Unit (BAP) of Nigde Omer Halisdemir University (NOHU), Nigde, Turkey, funding for this research work under project No. TGT 2020/17-LUTEP. The Ayhan Sahenk Foundation provided fellowship to MHH during his doctoral study.
dc.identifier.doi10.1007/s11627-022-10300-w
dc.identifier.endpage1076
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85140266200
dc.identifier.scopusqualityQ2
dc.identifier.startpage1066
dc.identifier.urihttps://doi.org/10.1007/s11627-022-10300-w
dc.identifier.urihttps://hdl.handle.net/11480/14746
dc.identifier.volume58
dc.identifier.wosWOS:000870613300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Plant
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectTomato
dc.subjectAgrobacterium transformation
dc.subjectEHA105
dc.subjectuidA
dc.subjectRio Grande
dc.titleEstablishment of highly efficient and reproducible Agrobacterium-mediated transformation system for tomato (Solanum lycopersicum L.)
dc.typeArticle

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