Effect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii

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dc.authoridInal, Muslum Suleyman/0000-0002-2836-6827
dc.contributor.authorInal, Muslum S.
dc.contributor.authorUnal, Dilek
dc.contributor.authorUnal, Bengu Turkyilmaz
dc.contributor.authorOzturk, Munir
dc.date.accessioned2024-11-07T13:34:07Z
dc.date.available2024-11-07T13:34:07Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPutrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis showed that polyamine metabolism induced gene expression at low-temperatures in the first 24 h. In the same way, the gene expression of heat shock proteins (hsp70c, hsp90a, and hsp90c) decreased under low-temperature treatment for 72 h; however, application of putrescine enhanced the gene expression in the first 24 h. The results obtained indicated that molecular response in the first 24 h could be important for cold acclimation. The psba and tba1 expressions were reduced under low-temperatures depending on the exposure time. In contrast, the exogenous putrescine enhanced the expression level of the psba response to low-temperature at 24 and 72 h. The results obtained in this study indicate that putrescine could play a role in the PS II repair mechanisms under low-temperature stress.
dc.description.sponsorshipBilecik Seyh Edebali University Research Foundation [2014-02-BIL-04-03]
dc.description.sponsorshipThis study was partially supported by the Bilecik Seyh Edebali University Research Foundation (2014-02-BIL-04-03) .
dc.identifier.doi10.32604/phyton.2022.018223
dc.identifier.endpage598
dc.identifier.issn0031-9457
dc.identifier.issn1851-5657
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85142659116
dc.identifier.scopusqualityQ3
dc.identifier.startpage583
dc.identifier.urihttps://doi.org/10.32604/phyton.2022.018223
dc.identifier.urihttps://hdl.handle.net/11480/15809
dc.identifier.volume91
dc.identifier.wosWOS:000712142100008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTech Science Press
dc.relation.ispartofPhyton-International Journal of Experimental Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectChlamydomonas reinhardtii
dc.subjectcold acclimation
dc.subjectheat shock protein
dc.subjectlow-temperature stress
dc.subjectPS II repair
dc.subjectputrescine
dc.titleEffect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii
dc.typeArticle

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