Expression of Jaburetox 2-Ec in Potato Encodes Resistance Against Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)

Basit Öğe Kaydı
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.contributor.authorTekinsoy, Merve
dc.contributor.authorBakhsh, Allah
dc.contributor.authorCaliskan, Mehmet Emin
dc.date.accessioned2024-11-07T13:31:42Z
dc.date.available2024-11-07T13:31:42Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe tomato leaf miner Tuta absoluta Meyrick is the most serious pest of potato worldwide. In the present study, we explored an alternate approach to induce resistance in potatoes against T. absoluta by engineering Jaburetox-2Ec, an insecticidal peptide derived from an isoform of urease from Jack bean (Canavalia ensiformis L.). The construct was developed by cloning a PCR-amplified fragment of Jaburetox-2Ec (270 bp) in a pBIN61 vector under the control of a 35S promoter and was named pBIN-JBTX. Furthermore, the recombinant plasmid expressing Jaburetox-2Ec was transformed into potato cv. Lady Olympia using Agrobacterium tumefaciens strain EHA105. The results showed the proper integration of the T-DNA region in primary transformants. Quantitative PCR assays showed the independent transgenic lines had enhanced transcript levels of Jaburetox. The leaf biotoxicity assays were performed by releasing pre-starved 2nd, 3rd, and 4th instar larvae of T. absoluta on transgenic potato plants. As a result, depending on the larval stage, the mortality rate of larvae feeding on transgenic plants ranged from 11 to 33%. Additionally, it was found that larvae feeding on control plants gained more weight than those feeding on transgenic plants. Besides that, deformities in larvae feeding on transgenic plants were also recorded. Overall, the results exhibited the insecticidal activity of Jaburetox 2-Ec against T. absoluta. The transgenic lines can be used as a source of germplasm for an efficient potato breeding programme.
dc.description.sponsorshipAyhan Sahenk Foundation; Council of Higher Education (CoHE)
dc.description.sponsorshipThe authors are grateful to the Ayhan Sahenk Foundation for providing a fellowship to Ms. Merve Tekinsoy during her master studies. Currently, Merve Tekinsoy is a PhD scholarship holder from the Council of Higher Education (CoHE) in the field of Plant Genetic and Agricultural Biotechnology, which is one of the 100 national priority areas determined by CoHE within the scope of the 100/2000 Program. The authors are also thankful to Dr. Claudio Ratti for providing pBIN61 vector and Prof. Dr. Celia R. Carlini and Dr. Fernanda Lopes for allowing to use Jaburetox-2Ec gene for research purpose. The authors are also grateful to Prof. Dr. Ayhan Gokce for providing advice related to entomological studies.
dc.identifier.doi10.1007/s11540-024-09788-z
dc.identifier.issn0014-3065
dc.identifier.issn1871-4528
dc.identifier.scopus2-s2.0-85204158949
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s11540-024-09788-z
dc.identifier.urihttps://hdl.handle.net/11480/14995
dc.identifier.wosWOS:001313278100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPotato Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectExpression
dc.subjectJaburetox
dc.subjectPlant urease
dc.subjectTuta absoluta
dc.titleExpression of Jaburetox 2-Ec in Potato Encodes Resistance Against Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu