Comparison of the effects of difenacoum and brodifacoum on the ultrastructure of rat liver cells

Basit Öğe Kaydı
dc.authorid0000-0003-3805-3215
dc.contributor.authorGul, Nursel
dc.contributor.authorYigit, Nuri
dc.contributor.authorSaygili, Fulya
dc.contributor.authorDemirel, Ebru
dc.contributor.authorGenis, Ceren
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractWe used transmission electron microscopy to examine the cytotoxic effects of the second-generation anticoagulant rodenticides difenacoum and brodifacoum on rat liver. A single dose of difenacoum or brodifacoum was administered to rats by gastric gavage and liver samples were taken after 24 h, four days or seven days. In the livers of rats treated with difenacoum for 24 h, hepatocytes typically showed increased numbers of lysosomes, as well as enlargement of both the perinuclear space and the cisternae of the rough endoplasmic reticulum (RER), while sinusoids were irregularly shaped and contained Kupffer cells. Similar irregularities occurred in brodifacoum-treated rats at the same time point, but additionally increased numbers of vacuoles, damaged mitochondrial cristae, and clumping of chromatin were observed in hepatocytes, and hemolysed erythrocytes were noted in the sinusoids. Comparable findings were made in each group of rats after four days. After seven days of difenacoum treatment, hepatocytes suffered loss of cytoplasmic material and mitochondrial shrinkage, while RER cisternae became discontinuous. In contrast, exposure to brodifacoum for seven days caused the formation of numerous vacuoles and lipid droplets, disordered mitochondrial morphology, chromatin clumping and invagination of the nuclear envelope in hepatocytes. Sinusoids in the livers of rodenticide-treated rats contained an accumulation of dense material, lipid droplets, cells with pycnotic nuclei and hemolysed erythrocytes. Overall, our results show that brodifacoum causes more severe effects in liver cells than difenacoum. Thus our microscopic data along with additional biochemical assays point to a severe effect of rodenticide on vertebrates.
dc.identifier.doi10.1515/aiht-2016-67-2783
dc.identifier.endpage209
dc.identifier.issn0004-1254
dc.identifier.issn1848-6312
dc.identifier.issue3
dc.identifier.pmid27749259
dc.identifier.scopus2-s2.0-85009772928
dc.identifier.scopusqualityQ3
dc.identifier.startpage204
dc.identifier.urihttps://dx.doi.org/10.1515/aiht-2016-67-2783
dc.identifier.urihttps://hdl.handle.net/11480/3576
dc.identifier.volume67
dc.identifier.wosWOS:000387052000004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherINST MEDICAL RESEARCH & OCCUPATIONAL HEALTH
dc.relation.ispartofARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecthepatocyte
dc.subjectrough endoplasmic reticulum (RER)
dc.subjectsinusoid
dc.subjecttransmission electron microscopy (TEM)
dc.subjectvacuole
dc.titleComparison of the effects of difenacoum and brodifacoum on the ultrastructure of rat liver cells
dc.typeArticle

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