Testing promising genes for pre-selection for drought tolerance in potato

Basit Öğe Kaydı
dc.authoridDemirel, Ufuk/0000-0002-3457-5086
dc.contributor.authorJameel, S.
dc.contributor.authorDemirel, U.
dc.contributor.authorGokce, Z. N. Ozturk
dc.date.accessioned2024-11-07T13:23:56Z
dc.date.available2024-11-07T13:23:56Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description30th International Horticultural Congress (IHC) / 2nd International Symposium on Root and Tuber Crops - Value Added Crops for the Next Generation -- AUG 12-16, 2018 -- Istanbul, TURKEY
dc.description.abstractPotato (Solanum tuberosum L.) is sensitive to drought mainly due to its fibrous root system. With the global climate change, the yield loss in potato cultivation caused by drought is expected to reach 30% over the following years. A breeding approach to increase the tolerance of potato to drought stress requires a long time, quite laborious and expensive, mainly due to limitations in a controlled drought treatment in the field conditions, high heterozygosity nature of the plant and genotype x environment (GxE) interaction. A pre-selection in the laboratory for the promising drought tolerant lines is a requirement to disentangle these obstacles. However, the complexity and multigenic nature of the drought stress response mechanism restrains the identification of molecular markers to be used in pre-selection purposes. Here, we used a novel approach and investigated the possibility of using the gene expression under controlled conditions for pre-selection of drought tolerant potato cultivars. For this purpose, we analyzed next generation sequencing results of 'Unica' (tolerant) and 'Russet Burbank' (sensitive) potato cultivars to 23 days of drought treatment and selected genes with contrasting expression levels under stress/control conditions. Control expression levels of five promising genes [plastidial pyruvate kinase 4 (XM_006360110.1), cryptochrome-2 (XM_006354425.1), cytosolic L-ascorbate peroxidase 1 (XM_006366063.1), early responsive to dehydration 15 (HG975443.1) and GAST1 (XM_006338257.1)] were tested by RT-PCR approach in a different set of potato genotypes with defined drought tolerance index evaluated in an independent field drought experiment to test their efficiency in pre-selection of drought tolerance potential. Although the results indicated the potential of plastidial pyruvate kinase 4 for detecting tolerant potato genotypes, it appears that the differentiation of tolerance and sensitivity potential by a simple RT-PCR approach still requires investigation of a large set of genes together.
dc.description.sponsorshipInt Soc Hort Sci
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [214O600]
dc.description.sponsorshipThis project study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) with project number 214O600.
dc.identifier.doi10.17660/ActaHortic.2019.1251.6
dc.identifier.endpage55
dc.identifier.isbn978-94-62612-48-8
dc.identifier.issn0567-7572
dc.identifier.issn2406-6168
dc.identifier.scopus2-s2.0-85073592217
dc.identifier.scopusqualityQ4
dc.identifier.startpage49
dc.identifier.urihttps://doi.org/10.17660/ActaHortic.2019.1251.6
dc.identifier.urihttps://hdl.handle.net/11480/13814
dc.identifier.volume1251
dc.identifier.wosWOS:000945548500006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherInt Soc Horticultural Science
dc.relation.ispartofXxx International Horticultural Congress (Ihc2018): Ii International Symposium on Root and Tuber Crops: Value Added Crops For the Next Generation
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectpotato
dc.subjectSolanum tuberosum L.
dc.subjectdrought stress
dc.subjectRT-PCR
dc.subjectbreeding
dc.titleTesting promising genes for pre-selection for drought tolerance in potato
dc.typeConference Object

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu