The effect of different doses of nonylphenol on the blood-testicular barrier integrity, hormone level, and DNA damage in the testes of rats

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dc.authoridBitgen, Nazmiye/0000-0002-6416-9230
dc.authoridCetindag, Emre/0000-0001-8063-8060
dc.authoridOz Gergin, Ozlem/0000-0002-1720-0194
dc.authoridOnder, Gozde Ozge/0000-0002-0515-9286
dc.contributor.authorGoktepe, Ozge
dc.contributor.authorOnder, Gozde Ozge
dc.contributor.authorCetindag, Emre
dc.contributor.authorBitgen, Nazmiye
dc.contributor.authorMat, Ozge Cengiz
dc.contributor.authorSuna, Pinar Alisan
dc.contributor.authorTufan, Emre
dc.date.accessioned2024-11-07T13:34:39Z
dc.date.available2024-11-07T13:34:39Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractDetermining the molecular characteristics of the damage caused by NP exposure in the testis is very important for understanding the source of the damage and developing treatment methods accordingly. Therefore, in this study, it is aimed to evaluate the toxic effects that different doses of NP may cause in the testis, including bloodtesticular barrier integrity and sperm DNA damage. For this purpose, 50 adult male Wistar albino rats were used in the study. Low, medium, and high-dose NP groups and the corn oil group were formed. After NP administration at determined doses for 15 days, the testis tissue taken under anesthesia was fixed in formaldehyde. Paraffin blocks were embedded using the routine histological tissue follow-up method. Histopathological and immunohistochemical analyses were performed by taking 5 mu m thick sections from paraffin blocks. The other testicular tissue was taken for the Western blot, Elisa, and comet methods, and the findings of sperm DNA analysis and the blood-testicular barrier were examined. NP caused the seminiferous epithelium to be disorganized and have significantly fewer cells in the testes of rats in different dose NP-induced groups. Compared with the control group, mTOR, Cx43, SCF, and HSP70 protein levels were decreased, while the expression of MMP-9 levels was increased in the different NP dose groups. Furthermore, tissue testosterone and inhibin B levels and SF-1 immunoreactivity intensity gradually decreased depending on the dose increase of NP. DNA damage of testicular tissues were increased in NP groups depending on NP dose. These results suggest that it is evident that NP, a commonly used industrial chemical, is an endocrine disrupting chemical (EDC) with estrogenic activity exerting adverse effects on health and that urgent measures are needed regarding the use.
dc.description.sponsorshipERCIYES University Scientific Research Project Unit [TCD-2021-11459]
dc.description.sponsorshipThis study was supported by the ERCIYES University Scientific Research Project Unit under number TCD-2021-11459.
dc.identifier.doi10.1016/j.fct.2023.113816
dc.identifier.issn0278-6915
dc.identifier.issn1873-6351
dc.identifier.pmid37164249
dc.identifier.scopus2-s2.0-85159081864
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.fct.2023.113816
dc.identifier.urihttps://hdl.handle.net/11480/16089
dc.identifier.volume177
dc.identifier.wosWOS:001001047700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofFood and Chemical Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectSpermatogenesis
dc.subjectCells
dc.subjectFate
dc.subjectReproduction
dc.subjectExpression
dc.subjectMaturation
dc.subjectApoptosis
dc.subjectReceptor
dc.subjectHsp70-2
dc.titleThe effect of different doses of nonylphenol on the blood-testicular barrier integrity, hormone level, and DNA damage in the testes of rats
dc.typeArticle

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