Detection of Theileria and Babesia species in sheep and goats by microscopy and molecular methods in Nigde province, Turkey

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dc.authoridAktas, Munir/0000-0002-3188-8757
dc.contributor.authorKaratepe, B.
dc.contributor.authorOzubek, S.
dc.contributor.authorKaratepe, M.
dc.contributor.authorAktas, M.
dc.date.accessioned2024-11-07T13:31:30Z
dc.date.available2024-11-07T13:31:30Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe present study was conducted to investigate presence and prevalence of Theileria and Babesia in sheep and goats of Nigde by microscopic and molecular methods. For this purpose, it was chosen a total of 690 small ruminants including 520 sheep and 170 goats between 1-10 year olds belonging to 28 flocks in Nigde province, in Central Anatolia of Turkey between April-July 2014. Blood smears were prepared from the blood samples, stained with Giemsa, and examined under the light microscope for Theileria and Babesia piroplasms. The piroplasm DNAs of Theileria and Babesia species was amplified using the RLB-F2 ve RLB-R2 primers. The polymerase chain reaction (PCR) products were hybridized to the membrane connected species-specific probes. The ticks were removed from sheep and goats and they were identified. Out of blood samples examined microscopically, 119 sheep (17.2%) were positive for Theileria spp. or Babesia spp., whereas no goats were positive. Of 690 animals 344 (49.8%) were positive for Theileria or Babesia by RLB method. Theileria ovis was the most common blood parasites in sheep and goats in the region with 46.9%, this is followed by B. ovis with 3.5% and Theileria sp. MK with 1.4%. The other Theileria species (T. lestoquardi, T. luwenshuni, T. uilenbergi) and genotypes (Theileria sp. OT1, Theileria sp. OT3) and Babesia species (B. motasi, B. crassa) were not detected in sheep and goats in the region. A total of 1361 ticks were collected in sheep and goats and they identified as Rhipicephalus bursa, R. turanicus, Hyalomma marginatum, Hy. excavatum, Hy. anatolicum, Haemophsalis parva, Hae. punctata, Hae. sulcata and Dermacentor marginatus. In determining blood parasites in sheep and goats, it was determined that PCR and RLB results were more susceptible than microscopy (P < 0.05,chi(2) = 105.823; P < 0.05,chi(2) = 164.548), and RLB than to PCR.
dc.description.sponsorshipScientific Research Projects Unit of Nigde Omer Halisdemir University [SSB 2015/01]
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Unit of Nigde Omer Halisdemir University (SSB 2015/01).
dc.identifier.endpage143
dc.identifier.issn0035-1555
dc.identifier.issn2258-0646
dc.identifier.issue7-9
dc.identifier.scopus2-s2.0-85087341917
dc.identifier.scopusqualityQ3
dc.identifier.startpage136
dc.identifier.urihttps://hdl.handle.net/11480/14875
dc.identifier.volume170
dc.identifier.wosWOS:000488552700004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherEcole Nationale Veterinaire Toulouse
dc.relation.ispartofRevue De Medecine Veterinaire
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectSheep
dc.subjectgoat
dc.subjectTheileria
dc.subjectBabesia
dc.subjecttick
dc.subjectmicroscopy
dc.subjectRLB
dc.titleDetection of Theileria and Babesia species in sheep and goats by microscopy and molecular methods in Nigde province, Turkey
dc.typeArticle

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