Development of Efficient, Reproducible and Stable Agrobacterium-Mediated Genetic Transformation of Five Potato Cultivars

Basit Öğe Kaydı
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.contributor.authorBakhsh, Allah
dc.date.accessioned2024-11-07T13:24:44Z
dc.date.available2024-11-07T13:24:44Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe developments in transformation technology have enabled the scientists to incorporate, mutate or substitute gene(s) leading to a particular trait; advancing it to a point where only few technical limitations remain. Genotype dependency and explant types are important factors affecting transformation efficiency in potato. In the present study, a rapid, reproducible and stable Agrobacterium-mediated transformation procedure in potato was developed by a combination of different plant growth regulators. Leaf discs and internodal explants of five cultivars of potato, i.e. Lady Olympia, Granola, Agria, Desiree and Innovator were infected with Agrobacterium tumefaciens strain LBA4404 containing pBIN19 expression vector with beta-glucuronidase gusA gene under the control of 355 CaMV promoter. Kanamycin was used as plant selectable markerfor screening of primary transformants at concentration of 100 mg/L. Both explants responded positively; internode being more suitable explant for better transformation efficiency. Based on GUS histochemical assay, the transformation efficiency was 22, 20, 18.6, 15 and 10% using the internodal explant, and 15, 12, 17, 8 and 6 % using leaf discs as explant in Lady Olympia, Granola, Agria, Desiree and Innovator respectively. Furthermore, PCR assays confirmed the presence of gusA and nptll genes in regenerated plants. The molecular analysis in succeeding progeny showed proper integration and expression of both genes. The results suggest Lady Olympia as the best cultivar for future transformation procedures. Overall, the short duration, rapidity and reproducibility make this protocol suitable for wider application of transgenic potato plants.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (Tubitak) [215O520, 115O022]
dc.description.sponsorshipThe present work was completed during research activities of projects (Project No. 215O520 and 115O022) supported by Scientific and Technological Research Council of Turkey (Tubitak). I would like to thank Tahira Hussain for her help in collecting data, Dr Halil Toktay for providing access to microscope facilities and Prof. Dr Mehmet Emin Caliskan for supplying seed tubers of cultivars used in the study. I am also thankful to Prof Dr Sebahattin Ozcan, University of Ankara, for providing recombinant plasmid used in the study.
dc.identifier.doi10.17113/ftb.58.01.20.6187
dc.identifier.endpage63
dc.identifier.issn1330-9862
dc.identifier.issn1334-2606
dc.identifier.issue1
dc.identifier.pmid32684788
dc.identifier.scopus2-s2.0-85085094097
dc.identifier.scopusqualityQ2
dc.identifier.startpage57
dc.identifier.urihttps://doi.org/10.17113/ftb.58.01.20.6187
dc.identifier.urihttps://hdl.handle.net/11480/14278
dc.identifier.volume58
dc.identifier.wosWOS:000530080000008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherFaculty Food Technology Biotechnology
dc.relation.ispartofFood Technology and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectpotato genetic transformation
dc.subjectgenotypes
dc.subjectplant growth regulators
dc.subjectcost-effective protocol
dc.titleDevelopment of Efficient, Reproducible and Stable Agrobacterium-Mediated Genetic Transformation of Five Potato Cultivars
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu