Trackable CEMB-Klean cotton transgenic technology: Affordable climate neutral agri-biotech industrialization for developing countries

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dc.contributor.authorQamar, Zahida
dc.contributor.authorTariq, Muhammad
dc.contributor.authorRehman, Tahir
dc.contributor.authorIqbal, Muhammad Shahzad
dc.contributor.authorSarwar, Muhammad Bilal
dc.contributor.authorSharif, Muhammad Nauman
dc.contributor.authorHassan, Zohaib
dc.date.accessioned2024-11-07T10:40:11Z
dc.date.available2024-11-07T10:40:11Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground: Transgenic technology reflects the incorporation of novel useful traits in crop plants like cotton for economic benefits by overcoming the problems including insects' pests and weeds in special. The present study is the success story of the continuous effort of CEMB team started back in the 1990s. Methods: This study includes characterization of a large number of Bacillus thuringiensis (Bt) strains taken from local soil and subjected to direct transformation of isolated BT genes into local cotton cultivars. Protocols for transformation into cotton plants were optimized and validated by the development of double gene codon optimized (CrylAc and Cry2A) transgenic cotton varieties. Results: The resulting GMOs in the form of CEMB-33, CA-12, CEMB-66 have been approved by Punjab Seed Council in 2013 and 2016 respectively. Double Bt and weedicide resistant cotton harboring CEMB-Modified and codon optimized cp4EPSPS (GTGene). These varieties can tolerate glyphosate spray @ 1900ml per acre without the appearance of necrotic spots/shedding and complete removal of all surrounding weeds in the cotton field is a significant advance to boost cotton production without spending much on insecticides and herbicides. Conclusion: In the current report, two unique sets of primers which amplify 1.1 Kb for CEMB-double Bt genes and 660 bp product for CEMB-Modified cp4EPSPS (GTGene) were tested. CEMB cotton variety CKC-01 is specially designed as low cost and easy to use by local farmer's technology has the potential to revolutionize the cotton growing culture of the country. © 2019 Centre of Excellence in Molecular Biology. All Rights Reserved.
dc.identifier.endpage138
dc.identifier.issn2310-5380
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85077446336
dc.identifier.scopusqualityQ3
dc.identifier.startpage131
dc.identifier.urihttps://hdl.handle.net/11480/11488
dc.identifier.volume6
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCentre of Excellence in Molecular Biology
dc.relation.ispartofAdvancements in Life Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectBt cotton
dc.subjectCEMB transgenic technology
dc.subjectEvent detection
dc.subjectGTGene
dc.titleTrackable CEMB-Klean cotton transgenic technology: Affordable climate neutral agri-biotech industrialization for developing countries
dc.typeArticle

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