Combined voltammetric and spectroscopic investigation of binding interaction between nifedipine and human serum albumin on polyelectrolyte modified ITO electrode

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dc.contributor.authorOzmen, Mustafa
dc.contributor.authorMaltas, Esra
dc.contributor.authorPatir, Imren Hatay
dc.contributor.authorBayrakci, Mevlut
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2013
dc.departmentNiğde ÖHÜ
dc.description.abstractThe binding interaction between the drug molecule, nifedipine (Nf), and the human serum albumin (HSA) on polyelectrolyte modified indium tin oxide (ITO) electrode has been investigated by the combination of electrochemical and fluorescence spectroscopy techniques. Surface modification has also been characterized by scanning electron microscopy (SEM) and Contact Angle (CA) measurements in each step. The cyclic voltammetry, electrochemical impedance parameters (peak potential difference (Delta E-p)), peak current difference (Delta I-p) and charge-transfer resistance (R-ct) indicate that nifedipine strongly interacted with human serum albumin molecule on the polyelectrolyte modified ITO electrode surface. Stern-Volmer quenching constant K-a is inversely correlated with the temperature, which indicates that the probable quenching mechanism of the nifedipine-human serum albumin binding reaction is initiated by complex formation. The results obtained from these techniques showed that Nf could bind to HSA. The binding constant (K-b) and the number of binding sites (n) of the drug with HSA at different temperatures were determined. At 298 K, K-b was found as 4.04 x 10(-3) and n was 1.08 for Nf-HSA. According to the van't Hoff equation, the thermodynamic parameters, Delta G, Delta H and Delta S, were obtained, showing the involvement of hydrophobic and electrostatic force in this interaction. (C) 2013 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipResearch Foundation of Selcuk University (BAP)
dc.description.sponsorshipWe would like to thank The Research Foundation of Selcuk University (BAP) for financial support of this work.
dc.identifier.doi10.1016/j.electacta.2013.08.130
dc.identifier.endpage542
dc.identifier.issn0013-4686
dc.identifier.issn1873-3859
dc.identifier.scopus2-s2.0-84883513861
dc.identifier.scopusqualityQ1
dc.identifier.startpage535
dc.identifier.urihttps://dx.doi.org/10.1016/j.electacta.2013.08.130
dc.identifier.urihttps://hdl.handle.net/11480/4321
dc.identifier.volume111
dc.identifier.wosWOS:000329531100069
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofELECTROCHIMICA ACTA
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectITO surface
dc.subjectPolyelectrolyte
dc.subjectAlbumin
dc.subjectNifedipine
dc.subjectInteraction
dc.titleCombined voltammetric and spectroscopic investigation of binding interaction between nifedipine and human serum albumin on polyelectrolyte modified ITO electrode
dc.typeArticle

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