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Öğe Antioxidant effects of propolis on carp Cyprinus carpio exposed to arsenic: biochemical and histopathologic findings(INTER-RESEARCH, 2014) Talas, Zeliha Selamoglu; Gulhan, Mehmet Fuat; Erdogan, Kenan; Orun, IbrahimPropolis, a resinous material produced by worker bees from the leaf buds and exudates of plants, is reported to possess various therapeutic properties. The aim of this study was to investigate the effects of propolis on biochemical parameters and histopathologic findings in carp Cyprinus carpio L. exposed to arsenic. A sublethal concentration of arsenic (0.01 mg l(-1)) and/or 10 mg l(-1) propolis were administered to fish for 1 wk. Catalase (CAT) activities and malondialdehyde (MDA) levels were determined in liver, gill and muscle tissues in control, arsenic only, propolis only and arsenic+propolis treatment groups. Results showed that CAT activity decreased in the arsenic group compared to the control and propolis groups. CAT activity in the arsenic+propolis group was significantly higher compared to the arsenic group. MDA levels in fish exposed to 0.01 mg l(-1) arsenic significantly increased compared to the control group. However, MDA levels in the arsenic+propolis group were significantly lower compared to the arsenic group. Histopathological changes in the liver, gill and muscle tissues of carp were examined by light microscopy: various changes were observed in all tissues of fish in the arsenic group. Propolis showed important antioxidant effects against arsenic toxicity in all fish tissues.Öğe Determination of some biochemical parameters of worker honeybees (Apis mellifera L.) belonging to different age groups(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2015) Akyol, Ethem; Selamoglu, Zeliha; Gulhan, Mehmet FuatThe catalase activity, total protein, and total RNA levels of worker bees of different age groups (4, 9, 15, 20, and 24 days old) within the same honeybee colony were studied. The catalase activities of group I (4 days), group II (9 days old), group III (15 days old), group IV (20 days old), and group V (24 days old) were 4.10 +/- 0.59, 4.32 +/- 0.57, 4.32 +/- 0.57, 4.41 +/- 0.49, and 4.47 +/- 0.48 kU/g, respectively. There were significant differences in total protein and total RNA levels among the different age groups of workers. The calculated total protein levels of group I, group II, group III, group IV, and group V were 22.28 +/- 0.77, 21.53 +/- 0.59, 20.95 +/- 1.05, 18.73 +/- 0.93, and 18.24 +/- 1.83 g/dL, respectively. The total RNA levels of group I, group II, group III, group IV, and group V were 22.42 +/- 0.16, 21.26 +/- 0.12, 19.30 +/- 0.08, 16.22 +/- 0.08, and 11.37 +/- 0.07 mu g/mu L, respectively. The results show that there was no significant difference among the average values of all age groups in catalase activity (P > 0.05), but significant differences were calculated among the total protein values of all age groups (P < 0.05).Öğe Effects of propolis, caffeic acid phenethyl ester, and pollen on renal injury in hypertensive rat: An experimental and theoretical approach(Wiley, 2017) Salmas, Ramin Ekhteiari; Gulhan, Mehmet Fuat; Durdagi, Serdar; Sahna, Engin; Abdullah, Huda I.; Selamoglu, ZelihaThe objective of this study was to evaluate the antioxidant effects of propolis, caffeic acid phenethyl ester (CAPE; active compound in propolis), and pollen on biochemical oxidative stress biomarkers in rat kidney tissue inhibited by N-nitro-L-arginine methyl ester (L-NAME). The biomarkers evaluated were paraoxonase (PON1), oxidative stress index (OSI), total antioxidant status (TAS), total oxidant status (TOS), asymmetric dimethylarginine (ADMA), and nuclear factor kappa B (NF-B). TAS levels and PON1 activity were significantly decreased in kidney tissue samples in the L-NAME-treated group (P<0.05). The levels of TAS and PONI were higher in the L-NAME plus propolis, CAPE, and pollen groups compared with the L-NAME-treated group. TOS, ADMA, and NF-B levels were significantly increased in the kidney tissue samples of the L-NAME-treated group (P<0.05). However, these parameters were significantly lower in the L-NAME plus propolis, CAPE, and pollen groups (P<0.05) compared with rats administered L-NAME alone (P<0.05). Furthermore, the binding energy of CAPE within catalytic domain of glutathione reductase (GR) enzyme as well as its inhibitory mechanism was determined using molecular modeling approaches. In conclusion, experimental and theoretical data suggested that oxidative alterations occurring in the kidney tissue of chronic hypertensive rats may be prevented via active compound of propolis, CAPE administration.Öğe Effects of various propolis concentrations on biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss)(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2009) Talas, Zeliha Selamoglu; Gulhan, Mehmet FuatBiochemical and hematological parameters in blood of rainbow trout treated to various concentrations of propolis for 96 h were determined. Total leukocyte count and granulocytes values increased (p < 0.05) in 0.02 and 0.03 g/L propolis groups. There was a decrease in agranulocytes (p < 0.05) erythrocytes, hemoglobin and hematocrit values for fish exposed to 0.02 and 0.03 g/L propolis. MCV and MCH values (p < 0.05) were significantly increased; 0.02 and 0.03 g/L propolis caused an increase (p < 0.05) in the levels of glucose, blood urea nitrogen, triglyceride, total cholesterol, lactate dehydrogenase, amylase and gamma glutamyltransferase. There was a decrease in the levels of aspartate aminotransferase and alkaline phosphatase. Hematological and biochemical protective effects of 0.01 g/L propolis were investigated. Dose-dependent effects of propolis on blood of fish can be favorable, opening new perspectives of investigation on their biological properties and utilization. (C) 2009 Elsevier Inc. All rights reserved.Öğe In-vitro Antioxidant Activities of the Ethanolic Extracts of Some Contained-Allantoin Plants(Shaheed Beheshti Univ, Sch Pharmacy, 2017) Selamoglu, Zeliha; Dusgun, Cihan; Akgul, Hasan; Gulhan, Mehmet FuatIt has been investigated the in-vitro antioxidant properties of ethanol extracts of the containedallantoin plants in this study. Contained-allantoined plant samples Plantago lanceolata, Plantago major, Robinia pseudoacacia, Platanus orientalis and Aesculus hippocastanum were tested at different concentrations. The antioxidant activities of plant samples were analysed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method, cupric reducing antioxidant capacity (CUPRAC), reducing power assay and beta-carotene bleaching method. Plantago major plant showed the highest antioxidant capacity compared to other plant extracts in results of the in-vitro assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method with 90.25 %, cupric reducing antioxidant capacity (CUPRAC) with 1.789 %, reducing power assay (FRAP) with 1.321 % and beta-carotene bleaching method with 78.01 % in 1 mg/mL. The lowest antioxidant activity was determined in Robinia pseudoacacia plant. In conclusion, allantoin shows antioxidant properties and it has the positive effect on total antioxidant capacity.Öğe Melatonin and Respiratory Diseases: A Review(BENTHAM SCIENCE PUBL LTD, 2017) Habtemariam, Solomon; Daglia, Maria; Sureda, Antoni; Selamoglu, Zeliha; Gulhan, Mehmet Fuat; Nabavi, Seyed MohammadMelatonin is an indoleamine with potent multifunctional biological and pharmacological effects, both receptor dependent and receptor-independent effects, including antioxidant, anticancer, antitumor, anti-inflammatory, anti-aging, anti-diabetic, antiviral, neuroprotective activities. Melatonin mitigates tissue injury via modification of abnormalities in redox status and other biochemical markers. At the molecular level, the biological and pharmacological activities of melatonin are attributed to the inhibition of nuclear factor-.appa beta (NF-kappa beta), c-Fos over expression and down-regulation of matrix metalloproteinases-3 (MMP-3), which are regulators of pro-inflammatory and pro-fibrotic cytokines. There are numerous scientific reports on the therapeutic potential of melatonin in treatment of asthma, respiratory diseases for infections, chronic obstructive pulmonary disease, lung cancer, pleural cavity diseases, as well as vascular pulmonary disease. In the present communication, we systematically review the therapeutic potential of melatonin in the treatment of respiratory diseases along with its molecular mechanism of actions.Öğe Physico-chemical and microbiological properties of water kefir produced from carob (Ceratonia siliqua L.) sherbet(Korean Society Food Science & Technology-Kosfost, 2024) Gulhan, Mehmet Fuat; Gulhan, Ayca; Dusgun, CihanIn this study, sugar-free (A) and sugar-added (B) formulations of carob sherbet were fermented with water kefir grains. After 48 h of fermentation, the pH and total soluble solid content level (TSS) of A and B decreased, while the titratable acidity (TA) value increased. At the end of fermentation, Ave B formulations were stored at 4 degrees C for 28 days. Fermented sherbets' 2,2 '-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity decreased with storage. A had 75.3% DPPH radical scavenging activity on day 0th, whereas B had 77.0%. B had less total phenolic content (TPC) than fermented A on day 0. TPC values decreased significantly, notably on days 21st and 28th of storage. After fermentation, A had more gallic acid, hesperidin, p-coumaric acid, rosmarinic acid, and syringic acid than B. A had 2.21% sucrose before fermentation and 0.95% after. On 14th days of storage, potassium and calcium had the greatest mineral levels. Compared to A, B gave higher microbial count values (Lactobacillus sp., Lactococcus sp. total mesophilic aerobic bacteria, and yeast) in the microbiological analysis results at the end of fermentation and during storage.Öğe Role of propolis on biochemical parameters in kidney and heart tissues against L-NAME induced oxidative injury in rats(INFORMA HEALTHCARE, 2014) Talas, Zeliha Selamoglu; Ozdemir, Ilknur; Ciftci, Osman; Cakir, Oguz; Gulhan, Mehmet Fuat; Pasaoglu, Oguz MuratNitric oxide (NO), produced by endothelial NO synthase, is recognised as a central antiinflammatory and antiatherogenic principle in the vasculature. Epidemiological and clinical studies have demonstrated that a growing list of natural products, as components of the daily diet or phytomedical preparations, may improve vascular function by enhancing NO bioavailability. In this article, we investigated antioxidant effects of propolis on biochemical parameters in kidney and heart tissues of acute NO synthase inhibited rats by N-omega-nitro-L-arginine methyl ester (L-NAME). There was increase (p < 0.001) in the activities of catalase and malondialdehyde levels in the L-NAME treatment groups when compared with control rats, but NO levels were decreased in both kidney and heart tissues. There were statistically significant changes (p < 0.001) in these parameters of L-NAME + propolis treated rats as compared with L-NAME-treated group. In summary, propolis may influence endothelial NO production.Öğe ROLE OF PROPOLIS ON OXIDATIVE STRESS IN VARIOUS TISSUES OF FISH(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2014) Aldemir, Osman Selcuk; Selamoglu, Zeliha; Gulhan, Mehmet Fuat; Cakir, Oguz; Ozdemir, Ilknur; Dastan, Sevgi Durna; Dogan, HusnuThe aim of this study was to investigate the therapeutic effects of propolis on biochemical parameters in tissues of rainbow trout (Oncorhynchus mykiss) exposed to cypermethrin. Cypermethrin is a potential toxic pollutant that directly threats the aquatic ecosystems and environment. Sublethal cypermethrin concentration of 0.0082 ppm was applied to the fish for 96 h. Propolis is a natural product collected from plants by honey bees. Its polyphenolic components, caffeic acid derivatives and flavonoids in particular, are matter of interest because of their strong antioxidant properties. Therapeutic concentration of propolis has been determined as 10 ppm in a previous study. Herein, malondialdehyde (MDA) levels and catalase (CAT) activities were determined in spleen and heart tissues of rainbow trouts. Results showed that CAT activities and MDA levels increased (P <= 0.001) in tissues of fish exposed to cypermethrin compared to control group. CAT activities and MDA levels in the tissues of fish decreased in cypermethrin+propolis group (P <= 0.001) compared to cypermethrin group. Propolis (10 ppm) on the tissues of the rainbow trout showed significant therapeutic effects. Propolis may serve as an antitoxic agent against pesticide toxicity to aquatic animals.Öğe Role of propolis on oxidative stress in various tissues of fish(Parlar Scientific Publications, 2014) Aldemir, Osman Selcuk; Selamoglu, Zeliha; Gulhan, Mehmet Fuat; Cakir, Oguz; Ozdemir, Ilknur; Dastan, Sevgi Durna; Dogan, HusnuThe aim of this study was to investigate the therapeutic effects of propolis on biochemical parameters in tissues of rainbow trout (Oncorhynchus mykiss) exposed to Cypermethrin. Cypermethrin is a potential toxic pollutant that directly threats the aquatic ecosystems and environment. Sublethal Cypermethrin concentration of 0.0082 ppm was applied to the fish for 96 h. Propolis is a natural product collected from plants by honey bees. Its polyphenolic components, caffeic acid derivatives and flavonoids in particular, are matter of interest because of their strong antioxidant properties. Therapeutic concentration of propolis has been determined as 10 ppm in a previous study. Herein, malondialdehyde (MDA) levels and catalase (CAT) activities were determined in spleen and heart tissues of rainbow trouts. Results showed that CAT activities and MDA levels increased (P?0.001) in tissues of fish exposed to Cypermethrin compared to control group. CAT activities and MDA levels in the tissues of fish decreased in cyperme-thrin+propolis group (P?0.001) compared to Cypermethrin group. Propolis (10 ppm) on the tissues of the rainbow trout showed significant therapeutic effects. Propolis may serve as an antitoxic agent against pesticide toxicity to aquatic animals.Öğe The Effects of Apitherapeutic Agents on Oxidative Stress in Serum Metabolic Parameters of Hypertensive Rats Created by Nitric Oxide Synthase Inhibited(Univ Kebangsaan Malaysia, 2021) Gulhan, Mehmet Fuat; Ozdemir, Betul; Selamoglu, Zeliha; Sahna, EnginHypertension is a chronic disease affecting the whole world due to its clinical effects and complications. The reduction in the release and effect of NO is one of the mechanisms of hypertension formation. Hypertension disrupts the balance between oxidant and antioxidant mechanisms. In this study, we aimed to observe biochemical changes in the blood by treatment propolis, caffeic acid phenethyl ester (CAPE) and pollen in hypertensive rats via Nco-Nitro-L-arginine methyl ester (L-NAME). Rats were divided in five groups. Rats were given L-NAME (40 mg/kg, intraperitoneally) for 14 days to make hypertensive. L-NAME and bee products were administered together with rats for 14 days, then L-NAME for 14 days. All administrated ended 28 days. There was a statistically significant (P<0.05) decrease in blood pressure (BP) in the groups in which bee products were applied. Blood pressure was lower in the pollen treated group than in the CAPE and propolis treated group (P<0.05). Paraoxanase (PON1), total antioxidant status (TAS), total oxidant status (TOS), asymmetric dimethylarginine (ADMA), nuclear factor-kappa B (NF-kappa B) levels were measured in the blood samples in all groups. In the L-NAME group; PON1, TAS, HDL, and total protein levels were found to be lower than the groups applied bee products (P<0.05). TOS, oxidative stress index (OSI), ADMA, NF-kappa B, glucose, cholesterol, LDL, friglyceride, ALT, AST, ALP levels were found to be lower in groups plus of bee products were applied compared to the group that received L-NAME (P<0.05). The results showed that oxidative stress and homeostasis can be regulated with propolis, CAPE and pollen in hypertensive rats induced L-NAME.Öğe The effects of pollen, propolis, and caffeic acid phenethyl ester on tyrosine hydroxylase activity and total RNA levels in hypertensive rats caused by nitric oxide synthase inhibition: experimental, docking and molecular dynamic studies(Taylor & Francis Inc, 2018) Salmas, Ramin Ekhteiari; Durdagi, Serdar; Gulhan, Mehmet Fuat; Duruyurek, Merve; Abdullah, Huda I.; Selamoglu, ZelihaThe objective of the present study was to evaluate the effects of propolis, pollen, and caffeic acid phenethyl ester (CAPE) on tyrosine hydroxylase (TH) activity and total RNA levels of N-nitro-L-arginine methyl ester (L-NAME) inhibition of nitric oxide synthase in the heart, adrenal medulla, and hypothalamus of hypertensive male Sprague dawley rats. The TH activity in the adrenal medulla, heart, and hypothalamus of the rats was significantly increased in the L-NAME group vs. control (p<0.05). Treatment with L-NAME led to a significant increase in blood pressure (BP) in the L-NAME group compared to control (p<0.05). These data suggest that propolis, pollen, and CAPE may mediate diminished TH activity in the heart, adrenal medulla, and hypothalamus in hypertensive rats. The decreased TH activity may be due to the modulation and synthesis of catecholamines and BP effects. In addition, the binding mechanism of CAPE within the catalytic domain of TH was investigated by means of molecular modeling approaches. These data suggest that the amino acid residues, Glu429 and Ser354 of TH may play a pivotal role in the stabilization of CAPE within the active site as evaluated by molecular dynamics (MD) simulations. Gibbs binding free energy (G(binding)) of CAPE in complex with TH was also determined by post-processing MD analysis approaches (i.e. Poisson-Boltzmann Surface Area (MM-PBSA) method).Öğe The investigation of antioxidant and anti-inflammatory potentials of apitherapeutic agents on heart tissues in nitric oxide synthase inhibited rats via N?-nitro-L-arginine methyl ester(Taylor & Francis Inc, 2021) Ozdemir, Betul; Gulhan, Mehmet Fuat; Sahna, Engin; Selamoglu, ZelihaBackground High blood pressure effects heart and vessels. Development of pathogenesis is the result of oxidative stress. We aimed to investigate the antioxidant effects of propolis, caffeic acid phenethyl ester (CAPE), and pollen on the hearts of rats which chronic nitric oxide synthase (NOS) inhibited through N omega-nitro-L-arginine methyl ester (L-NAME). Paraoxonase 1 (PON1), total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), asymmetric dimethylarginine (ADMA), and nuclear factor-kappa B (NF-kappa B) were analyzed on the heart. Material and Methods Sprague-Dawley rats were divided five groups of seven rats in every group; Group I: Control, Group II: L-NAME, Group III: L-NAME+propolis, Group IV: L-NAME+CAPE and Group V: L-NAME+pollen. L-NAME become dissolved in regular saline (0.9% NaCl w/v). The ethanolic extract of propolis (200 mg/kg/days, gavage), pollen (100 mg/kg/days, by gavage), CAPE (50 mu M/kg/days, intraperitoneally), and the NOS inhibitor L-NAME (40 mg/kg, intraperitoneally) had been administered. Results Blood pressure (BP) of rats treated with propolis, CAP,E and pollen statistically significant decreased. Decreasing in BP of the rats of pollen group was more than CAPE and propolis groups (P< .05). PON1 and TAS levels decreased in L-NAME-treated groups (P< .05), but ranges have been better in propolis, CAPE and pollen groups. TOS, ADMA and NF-kappa B levels increased (P< .05) in L-NAME group; however, these parameters were lower (P< .05) in propolis and CAPE groups (P< .05). Conclusions Vasorelaxant properties and free radical scavenging actions of propolis, CAPE, and pollen may reduce the oxidative stress and blood pressure in the rats chronic NOS inhibited through L-NAME.
