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    AN EFFICIENT AND COST EFFECTIVE STERILIZING METHOD WITH LEAST MICROBIAL CONTAMINATION AND MAXIMUM GERMINATION RATIO FOR IN VITRO COTTON (Gossypium hirsutum L.) CULTURE
    (PAKISTAN AGRICULTURAL SCIENTISTS FORUM, 2016) Bakhsh, A.; Anayol, E.; Sancak, C.; Ozcan, S.
    Cotton (Gossypium hirsutum L.) is a highly significant crop because of its quality for textile industry of the world. With the advent of genetic engineering, cotton has gone through several genetic improvements that encode tolerance against biotic and abiotic stresses. The efficient seed sterilization method is essential for successful genetic improvement in cotton. The present study was conducted to compare different sterilizing agents employed for in vitro culture of cotton to find out an efficient, reproducible, cost effective and genotype independent in vitro seed sterilization procedure of cotton. The two cultivated cotton cultivars i.e. GSN-12 and Coker-312 were used for these studies that were further transformed with insecticidal genes under different promoters. The different sterilizing agents used (in separate or combination) were ethanol, commercial bleach (NaOCl), mercuric chloride (HgCl2), Sodium Dodecyle (SDS), hydrogen peroxide (H2O2). The surface sterilized seeds of both cultivars were cultured on MS medium and data was recorded for contamination free seeds and germination ratio. The results showed that 5% n-Hexane in combination with 2% of H2O2 are the best sterilizing agents for both cultivars when compared to the efficacy of other chemicals. Seeds treated with n-hexane and hydrogen peroxide had minimum microbial contamination with improved germination rate. The sterilizing method was successfully used for surface sterilization of other cotton cultivars in our project. According to our knowledge, this is the first report of using n-hexane in combination with H2O2 for surface sterilization of cotton seed for in vitro culture studies.
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    Elimination of Unnecessary Protein Production of Transgenes in Transgenic Plants
    (SPRINGER, 2014) Ozcan, S.; Anayol, E.; Bakhsh, A.; Ozcan, S.; Aasim, M.; Gurbuz, B.; Unlu, L.
    [Abstract Not Available]
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    Evaluation of Jaw Bone Change in Children and Adolescents with Rheumatic Heart Disease by Fractal Analysis
    (Wolters Kluwer Medknow Publications, 2024) Temur, K. T.; Magat, G.; Cosgunarslan, A.; Ozcan, S.
    Background: Recognizing the oral needs of people living with rheumatic heart disease (RHD), particularly in relation to their jaws, is of paramount importance. Aims: This study aimed to evaluate potential jaw bone changes in children and adolescents diagnosed with RHD and compare them with a group of healthy children and adolescents. Methods and Material: The dental panoramic radiographs (DPRs) of 32 children and adolescents diagnosed with RHD comprised the case group. In contrast, the control group consisted of DPRs from 32 children and adolescents without any known diseases. Two regions of interest (ROI1 and ROI2) of trabecular bone and a separate region of interest (ROI3) of cortical bone were selected for fractal analysis. The mandibular cortical index (MCI) was evaluated. Results: The ROI1 fractal dimension (FD) value of the individuals in the case group was statistically higher in the right jaw than in the left jaw (P < 0.01). Significant differences were determined between the case and control groups regarding the left ROI1, and the left and the right ROI2 (P < 0.05). No significant difference was found between the patient and control groups regarding the MCI value (P > 0.05). Conclusions: The results of this study showed that bone metabolism was affected unilaterally in the condyle region and bilaterally in the gonial region of patients with RHD. It also revealed that the cortical layer was not affected.