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Öğe Aluminum Toxicity: A Case Study on Tobacco (Nicotiana tabacum L.)(Tech Science Press, 2023) Ozturk, Munir; Metin, Mert; Altay, Volkan; Kawano, Tomonori; Gul, Alvina; Unal, Bengu Turkyilmaz; Unal, DilekAluminum is an abundant metal in the earth's crust that turns out to be toxic in acidic environments. Many plants are affected by the presence of aluminum at the whole plant level, at the organ level, and at the cellular level. Tobacco as a cash crop (Nicotiana tabacum L.) is a widely cultivated plant worldwide and is also a good model organism for research. Although there are many articles on Al-phytotoxicity in the literature, reviews on a single species that are economically and scientifically important are limited. In this article, we not only provide the biology associated with tobacco Al-toxicity, but also some essential information regarding the effects of this metal on other plant species (even animals). This review provides information on aluminum localization and uptake process by different staining techniques, as well as the effects of its toxicity at different compartment levels and the physiological consequences derived from them. In addition, molecular studies in recent years have reported specific responses to Al toxicity, such as overexpression of various protective proteins. Besides, this review discusses data on various organelle-based responses, cell death, and other mechanisms, data on tobacco plants and other kingdoms relevant to these studies.Öğe Ameliorative effect of Halopteris filicina extracts on growth parameters and genomic DNA template stability of tomato (Solanum lycopersicum) under lead chloride stress(Csiro Publishing, 2022) Unal, Dilek; Sevim, Gulcin; Varis, Gokay; Tuney-Kizilkaya, Inci; Unal, Bengu Turkyilmaz; Ozturk, MunirLead is a toxic element that accumulates in agricultural soils through various anthropogenic sources. It inhibits the growth and development of plants and causes mutations in DNA. Macroalgae such as Halopteris filicina contain multifunctional components that may improve plant tolerance to lead stress. In this study, seeds of tomato (Solanum lycopersicum) were subjected to six treatments comprising two levels of lead exposure (60 or 120 mu M PbCl2) with or without H. filicina extract (0.5% in distilled water), a distilled water control, and a positive control (H. filicina extract) for 7 days. Physiological responses were investigated. Seedlings that had been treated with 60 and 120 mu M PbCl2 without H. filicina extract showed root growth reduction of 55% and 68.6%, respectively, relative to the control, whereas for 60 and 120 mu M PbCl2-treated seedlings with H. filicina extract applied, the reductions in root growth were lower, at 27.44% and 50.51%. The seedling viability index was decreased by 68.14% at 120 mu M PbCl2 application without H. filicina extract, whereas a 42.48% reduction was recorded for 120 mu M PbCl2-treated seedlings with H. filicina extract applied. Moreover, PbCl2 accumulation resulted in a decrease in leaf pigment content. Leaf pigment content was high in plants receiving the H. filicina extract. The rate of lipid peroxidation caused by PbCl2 was reduced with application of H. filicina extract. Genomic template stability was determined by using the inter simple sequence repeat-PCR technique, which revealed a decrease in DNA stabilisation with an increase in lead accumulation. However, this was alleviated by application of H. filicina extract. Our findings indicate that H. filicina extract both stimulates plant growth and protects from toxic effects by reducing accumulation of metals in the cell.Öğe An Overview of Olive Cultivation in Turkey: Botanical Features, Eco-Physiology and Phytochemical Aspects(Mdpi, 2021) Ozturk, Munir; Altay, Volkan; Gonenc, Tuba Mert; Unal, Bengu Turkyilmaz; Efe, Recep; Akcicek, Eren; Bukhari, AndleebGlobal climate change, especially global warming, is affecting olive production efficiency as well as its product quality. The size and water content of fruit varies depending on the olive fruit yield along with the region, climate, and geographical position as well as agricultural applications. Anthropogenic activities also affect its ecology to a great extent. The plant prefers areas with mild winters and short rainy seasons but is facing long and dry summers, sunny habitats, well drained dry, poor, loamy, clayey-loamy, slightly calcareous, pebbly and nutrient-rich soils, with a pH around 6-8. It is resistant to drought but suffers much from harsh winters and air pollutants, which affect its production. Although the olive plant tolerates temperatures between -7 degrees C to 40 degrees C, the optimum temperature demanded for growth, development, and fruit yield is 15-25 degrees C. The annual precipitation demand lies between 700-850 mm. An important part of the composition of its fruit consists of water and oil or the liquid gold. Main ingredients are additionally fat-soluble vitamins, minerals, organic sugars, and phenolics. Phenolic substances are responsible for many beneficial health effects as well as the taste and aroma of olive fruit. Oleuropein stands out due to its inhibition of oxidation of low density lipoproteins and its hypoglycemic and cholesterolemic effects. It is also a component that protects the olive tree against various parasites and diseases, one of the reasons why olive is recorded as the immortal tree. Olive trees are cultivated in different regions of Turkey. A series of changes occur in morphological, physiological, and biochemical features to overcome different types of stress. In this review, information about the botanical aspects, eco-physiology, and pharmaceutical features of the oil, fruit, and leaves has been evaluated.Öğe Arsenic and Human Health: Genotoxicity, Epigenomic Effects, and Cancer Signaling(Springernature, 2022) Ozturk, Munir; Metin, Mert; Altay, Volkan; Bhat, Rouf Ahmad; Ejaz, Mahnoor; Gul, Alvina; Unal, Bengu TurkyilmazArsenic is a well-known element because of its toxicity. Humans as well as plants and animals are negatively affected by its exposure. Some countries suffer from high levels of arsenic in their tap water and soils, which is considered a primary arsenic-linked risk factor for living beings. Humans generally get exposed to arsenic by contaminated drinking waters, resulting in many health problems, ranging from cancer to skin diseases. On the other hand, the FDA-certified drug arsenic trioxide provides solutions for various diseases, including several types of cancers. This issue emphasizes the importance of speciation of the metalloid elements in terms of impacts on health. When species get exposed to arsenic, it affects the cells altering their involvement. It can lead to abnormalities in inflammatory mechanisms and the immune system which contribute to the negative impacts generated on the body. The poisoning originating from arsenic gives rise to various biological signs on the body which can be useful for the diagnosis. It is important to find true biomarkers for the detection of arsenic poisoning. In view of its application in medicine and biology, studies on understanding the biological activity of arsenic have increased. In this review, we aim at summarizing the current state of knowledge of arsenic and the mechanism behind its toxicity including genotoxicity, oxidative insults, epigenomic changes, and alterations in cellular signaling.Öğe Biotechnological interventions for removal of heavy metals and metalloids from water resources(wiley, 2023) Ozturk, Munir; Unal, Bengu Turkyilmaz; Turker, HuseyinUsable water resources, which are essential for the survival of living beings, are getting severely polluted due to rapid population growth, industrialization, urbanization, chemicals used in agriculture as well as other human interventions. Polluted water resources affect plants and microorganisms, thereby posing a great threat to animal and human health throughout the food chain. One of the most important factors involved in the pollution of our waters is the accumulation of heavy metals and metalloids, as their acute toxic effects should be overcome and these must be converted into less toxic forms following appropriate methods. In recent years, biotechnological methods are being preferred due to their lower cost and environmental friendliness as compared to the chemical and physical methods evaluated for the removal of heavy metals and metalloids. Some of these methods include biodegradation, microbial biosorption, mycoremediation, cyanoremediation, phytoremediation, and biosparging. The use of biological materials such as bacteria, cyanobacteria, fungi, lichens, algae, and plants has become one of the preferred methods for the decontamination of our polluted waters. The biosensors are also used to detect metal and metalloid accumulation in waters and have proved useful tools for the early detection of pollution and taking precautionary measures. This chapter will enlighten the latest developments and the applications in this connection. © 2024 John Wiley & Sons Ltd. All rights reserved.Öğe Effect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii(Tech Science Press, 2022) Inal, Muslum S.; Unal, Dilek; Unal, Bengu Turkyilmaz; Ozturk, MunirPutrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis showed that polyamine metabolism induced gene expression at low-temperatures in the first 24 h. In the same way, the gene expression of heat shock proteins (hsp70c, hsp90a, and hsp90c) decreased under low-temperature treatment for 72 h; however, application of putrescine enhanced the gene expression in the first 24 h. The results obtained indicated that molecular response in the first 24 h could be important for cold acclimation. The psba and tba1 expressions were reduced under low-temperatures depending on the exposure time. In contrast, the exogenous putrescine enhanced the expression level of the psba response to low-temperature at 24 and 72 h. The results obtained in this study indicate that putrescine could play a role in the PS II repair mechanisms under low-temperature stress.Öğe Effect of survey farmers’ knowledge and practices on the yield of sugarcane in Pakistan(King Saud University, 2023) Khan, Faheem; Huma, Zelle; Shah, Ghulam Abbas; Turkyilmaz Unal, Bengu; Ozturk, MunirSugarcane (Saccharum interspecific hybrids) yield is greatly affected by different pests in Pakistan. A total of three hundred and thirty-six (336) volunteer sugarcane farmers were interviewed and their fields inspected. Major pests were noted as; rat (Bandicota bengalensis), termites (Heterotermes indicola), borer (Bissetia steniellus), and leafhopper (Pyrilla perpusilla). These occurred in single and combined status in sugarcane fields. Most (147/336) of the sugarcane farmers mentioned four types of pests in their fields. In general 200/336 of the sugarcane farmers had applied pesticides in single and combined forms in their fields, they achieved a maximum average yield of 80 t ha?1. The number of farmers who applied malathion was 36/336. They achieved an average yield of 105.5 kg ha?1. Only one sugarcane farmer applied regent and furadon in combined form and obtained an average yield of 92.6 kg ha?1 in their field. Linear regression showed a significant relationship between average sugarcane yield and pest infestation (R2 = 0.9689) as well as pesticide application (R2 = 0.9128) in the area investigated. In total 299 out of the 336 sugarcane farmers were small size landholders up to 2 ha. A limited number (35/336) of the farmers visited the public agricultural extension department for information and application techniques of pesticides against pests. It will be fruitful if the public and private agricultural extension departments start awareness programmes on pest infestation among sugarcane farmers and train them to practice recommended pesticide applications in their sugarcane fields. © 2022 The AuthorsÖğe EFFECTS OF THE EXTRACT FROM DIFFERENT PLANT PARTS OF FERULA COMMUNIS SPP. COMMUNIS ON THE SEED GERMINATION AND SEEDLING GROWTH OF BARLEY AND CUCUMBER(Parlar Scientific Publications (P S P), 2018) Turkyilmaz-Unal, Bengu; Esiz-Dereboylu, Aylin; Guvensen, Aykut; Tort, Nedret; Ozturk, MunirThe present study was conducted to observe the effects of Ferula communis L. ssp. comntunis on seed germination and seedling growth of barley and cucumber. Different concentrations of the extract showed inhibition in all parameters. The maximum reduction was recorded in the germination of barley (60 %) and a lowest vigor index noted when 50 percent leaf extract was applied. The maximum decrease in root (91.88 %) and shoot (82.46 %) length in the barley seedlings occurred in 50 percent flower extract. The maximum reduction in root fresh weight (80.28 %), dry weight (71.43 %) and shoot fresh weight (80.33 %) was found in 50 percent flower extract application and whole plant extract was responsible for 50 percent reduction in the shoot dry weight of barley seedlings. No germination in cucumber was observed in 50 percent flower extract application. The maximum reduction of germination rate (53 %) and vigor index in other groups was again around 50 percent with fruit extract application. The greatest reduction was recorded in root (62.16 %) and shoot (27.55 %) length, root fresh (75.86 %) and dry (36.59 %) weights, and shoot fresh (56.76 %) and dry (13.64 %) weights in cucumber seedlings after applying 50 percent leaf extract. Roots were more affected than shoots and the extracts taken from flowers and leaves were more suppressive as compared to the fruits and whole plant.Öğe Exogenous application of phytohormones and phytometabolites to plants to alleviate the effects of drought stress(Elsevier, 2023) Ozturk, Munir; Unal, Bengu TurkyilmazAs sessile organisms, plants have to cope with various stress factors in their environment. Drought is the most widespread abiotic stress factor and plants may undergo some morphological, cellular, physiological, biochemical, and transcriptomics changes in alleviating it. When exposed to drought, many primary and secondary metabolites with osmoprotectant or antioxidant properties as well as phytohormones are synthesized. Phytohormones and phytometabolites are thought to play a key role in development and yield in plants under drought stress. With the exogenous application of these, the amounts of endogenous phytohormones and phytometabolites increase, and the protection rate of plants from the effects of drought improved. This chapter highlights recent developments in drought tolerance caused by exogenous applications. © 2023 Elsevier Inc. All rights reserved.Öğe Genome modifications in cassava(Elsevier, 2024) Imran, Hajira; Baig, Danish Ilyas; Jabbar, Warda; Gul, Alvina; Gul, Warda; Ozturk, Munir; Turkyilmaz Unal, BenguCassava (Manihot esculenta Crantz), a tropical crop, is consumed as a second major source of carbohydrates worldwide. Its origin traces back to Africa, and then it flourished throughout the world by becoming the staple food for more than half of the world’s population. Nowadays, this crop has become a center of research because of its economic and nutritional value. Besides having many attributes, cassava also needs improvement in various aspects, i.e., breeding practices, tolerance to abiotic stresses, disease resistance, and enhanced starch content. This has been improved by using gene editing technologies like TALENs (transcription activator-like effector nucleases), ZFNs (zinc finger nucleases), mega nucleases, and CRISPRs (clustered regularly interspaced short palindromic repeats) associated with Cas-9 protein. In addition to re-evaluating the importance of these strategies in cassava improvement, this manuscript also includes the biofortification, modification, and storage of starch, as well as the root production and postharvest storage of cassava. © 2024 Elsevier Inc. All rights reserved.Öğe Halophytes have potential as heavy metal phytoremediators: A comprehensive review(Pergamon-Elsevier Science Ltd, 2022) Caparros, Pedro Garcia; Ozturk, Munir; Gul, Alvina; Batool, Tuba Sharf; Pirasteh-Anosheh, Hadi; Unal, Bengu Turkyilmaz; Altay, VolkanHalophytes are widely distributed worldwide and thrive in a wide range of environments such as coastal salt marshes, dunes, saline depressions, and inland deserts. They are also able to cope with heavy metal stress due to their developed morphological and physiological traits such as restricted entry of heavy metals through the root system, synthesis and storage of osmolytes such as proline, and intracellular complexation/chelation/compartmentalization of metal ions. Heavy metal-polluted areas result in yield losses of crops, higher environmental risks for the population, and a severe reduction in biodiversity of these areas. This review highlights studies on the heavy metal phytoremediation capacity of halophytic species from different plant families. An attempt has been made to include the pertinent information regarding heavy metal phytoremediation together with the most important characteristics of halophytes, followed by information on different studies conducted under controlled conditions or in natural environments related to heavy metal phytoremediation of different halophyte taxa from different families. The most promising species have been classified as phytoextractors or phytostabilizers together with recommendations for future research prospects in this topic.Öğe Molecular Biology of Cadmium Toxicity in Saccharomyces cerevisiae(Springernature, 2021) Ozturk, Munir; Metin, Mert; Altay, Volkan; De Filippis, Luigi; Unal, Bengu Turkyilmaz; Khursheed, Anum; Gul, AlvinaCadmium (Cd) is a toxic heavy metal mainly originating from industrial activities and causes environmental pollution. To better understand its toxicity and pollution remediation, we must understand the effects of Cd on living beings. Saccharomyces cerevisiae (budding yeast) is an eukaryotic unicellular model organism. It has provided much scientific knowledge about cellular and molecular biology in addition to its economic benefits. Effects associated with copper and zinc, sulfur and selenium metabolism, calcium (Ca2+) balance/signaling, and structure of phospholipids as a result of exposure to cadmium have been evaluated. In yeast as a result of cadmium stress, mitogen-activated protein kinase, high osmolarity glycerol, and cell wall integrity pathways have been reported to activate different signaling pathways. In addition, abnormalities and changes in protein structure, ribosomes, cell cycle disruption, and reactive oxygen species (ROS) following cadmium cytotoxicity have also been detailed. Moreover, the key OLE1 gene that encodes for delta-9 FA desaturase in relation to cadmium toxicity has been discussed in more detail. Keeping all these studies in mind, an attempt has been made to evaluate published cellular and molecular toxicity data related to Cd stress, and specifically published on S. cerevisiae.Öğe Nanotechnologies for environmental remediation and their ecotoxicological impacts(Springer, 2023) Ejaz, Mahnoor; Gul, Alvina; Ozturk, Munir; Hafeez, Ahmed; Turkyilmaz Unal, Bengu; Jan, Sami Ullah; Siddique, Muhammad TaimurEnvironmental nanoremediation is an emerging technology that aims to rapidly and efficiently remove contaminants from the polluted sites using engineered nanomaterials (ENMs). Inorganic nanoparticles which are generally metallic, silica-based, carbon-based, or polymeric in nature serve to remediate through chemical reactions, filtration, or adsorption. Their greater surface area per unit mass and high reactivity enable them to treat groundwater, wastewater, oilfields, and toxic industrial contaminants. Despite the growing interest in nanotechnological solutions for bioremediation, the environmental and human hazard associated with their use is raising concerns globally. Nanoremediation techniques when compared to conventional remediation solutions show increased effectivity in terms of cost and time; however, the main challenge is the ability of ENMs to remove contaminants from different environmental mediums by safeguarding the ecosystem. ENMs improving the accretion of the pollutant and increasing their bioavailability should be rectified along with the vigilant management of their transfer to the upper levels of the food chain which subsequently causes biomagnification. The ecosystem-centered approach will help monitor the ecotoxicological impacts of nanoremediation considering the safety, sustainability, and proper disposal of ENMs. The environment and human health risk assessment of each novel engineered nanomaterial along with the regulation of life cycle assessment (LCA) tools of ENMs for nanoremediation can help investigate the possible environmental hazard. This review focuses on the currently available nanotechnological methods used for environmental remediation and their potential toxicological impacts on the ecosystem.Öğe Osmoregulation and its actions during the drought stress in plants(Wiley, 2021) Ozturk, Munir; Turkyilmaz Unal, Bengu; Garcia-Caparros, Pedro; Khursheed, Anum; Gul, Alvina; Hasanuzzaman, MirzaDrought stress, which causes a decline in quality and quantity of crop yields, has become more accentuated these days due to climatic change. Serious measures need to be taken to increase the tolerance of crop plants to acute drought conditions likely to occur due to global warming. Drought stress causes many physiological and biochemical changes in plants, rendering the maintenance of osmotic adjustment highly crucial. The degree of plant resistance to drought varies with plant species and cultivars, phenological stages of the plant, and the duration of plant exposure to the stress. Osmoregulation in plants under low water potential relies on synthesis and accumulation of osmoprotectants or osmolytes such as soluble proteins, sugars, and sugar alcohols, quaternary ammonium compounds, and amino acids, like proline. This review highlights the role of osmolytes in water-stressed plants and of enzymes entailed in their metabolism. It will be useful, especially for researchers working on the development of drought-resistant crops by using the metabolic-engineering techniques.Öğe Phytohormones and Stress Responsive Secondary Metabolites(Elsevier, 2023) Ozturk, Munir; Bhat, Rouf Ahmad; Ashraf, Muhammad; Tonelli, Fernanda Maria Policarpo; Unal, Bengu Turkyilmaz; Dar, Gowhar HamidPhytohormones and Stress Responsive Secondary Metabolites provides a deep dive into the signaling pathways associated with phytohormones and phytometabolites. With a strong focus on plant stress responses and DNA technology, the book highlights plant biotechnology and metabolic engineering principles. Biotechnology, by using DNA editing technologies, allows the expression of plant genes into other plant species with desirable modulation on plant behavior. Beginning with an overview of phytohormone signaling, growth and abiotic and biotic stresses, subsequent chapters explore DNA modification strategies, epigenetic and epigenomic regulation, and miRNA regulation. This book will be an essential resource for students, researchers and agriculturalists interested in plant physiology, plant genetics and plant biotechnology. © 2023 Elsevier Inc. All rights reserved.Öğe PHYTOTOXICITY OF ABOVE - GROUND WEED RESIDUE AGAINST SOME CROPS AND WEEDS(Pakistan Botanical Soc, 2020) Mushtaq, Waseem; Mehdizade, Mohammad; Siddiqui, Mohammad Badruzzaman; Ozturk, Munir; Jabran, Khawar; Altay, VolkanWeed residues mixing with the soil after the death of weeds can inhibit growth and development of crop plants. A study was conducted to assess the allelopathic intrusion of Nicotiana plumbaginifolia above-ground residues on growth of selected crops (Pisum sativum, Cicer arietinum) and weeds (Cassia tora, C. sophera), nature of chemicals involved, role of nutrients and physico-chemical parameters. Growth responses of weeds and crops were analyzed for soil amended with different rates of Nicotiana residue (residue amended soil, RS) and residual extract (residue extract amended soil, RES). Likewise, the growth behaviour of test plants was also evaluated against extract of residue in hydroponics (residue extract, RE). Physiochemical screening of amended soils was conducted to look for concentration and bioavailability of essential nutrients. The soil amended with residues (RS and RES) showed inhibitory effect on test plants, however, residue extract (RE) had more inhibitory effects. The inhibition in growth (root length, shoot length and dry biomass) was concentration dependent and C. tora plants experienced the highest reduction among all test plants. A partial enrichment was observed in nutrient status and phenolic content as we increased the concentration of the soil amendment or the extract. This points out that growth inhibition occurred due to phenolic and not due to nutrient depletion. These findings indicate a role of putative phenolic allelochemicals forced allelopathic effects on C. arietinum, P. sativum, C. tora and C. sophera.Öğe Role of Rare Earth Elements in Plants(Springer, 2023) Ozturk, Munir; Metin, Mert; Altay, Volkan; Prasad, Majeti Narasimha Vara; Gul, Alvina; Bhat, Rouf Ahmad; Darvash, Moonisa AslamRare earth elements (generally abbreviated as REEs) are the name used to define 17 metals with special physicochemical features. In general, REEs are interest of chemists mainly because of their peculiar chemical abilities. However, this situation started to change, and REEs, recently, turn out to be a hotspot also for environmental biologist, plant biologist, and molecular biologists. Despite that there are diverse studies regarding biology of these elements (also defined as metals), biologist still have limited knowledge about the mechanisms of REE action in living (particular in reducing their toxic effects at high doses) and about the areas in which these metals can be used as biotechnological tools. REEs have a peculiarity that they can bind to other molecules to enhance several physiological activities like growth and development in plants and photosynthesis, and they are able to behave as synergistic agents for the intake of several nutrients. The supply of these elements in several species can be, as well, an important source of synthesis of natural compounds. The exogenous application of REEs in plants has been demonstrated to antagonize damages of salinity and metal stresses. The present review aims to put forward a comprehensive account of the latest findings related to the effects of REEs' on different aspects of plant growth and development. This compilation mainly targets scientists who afford to discover action mechanisms of REEs and researchers focussing on the amelioration of adverse consequences generated by REEs.Öğe VARIATIONS IN THE PROLINE AND TOTAL PROTEIN CONTENTS IN ORIGANUM SIPYLEUM L. FROM DIFFERENT ALTITUDES OF SPIL MOUNTAIN, TURKEY(PAKISTAN BOTANICAL SOC, 2013) Unal, Bengu Turkyilmaz; Guvensen, Aykut; Dereboylu, Aylin Esiz; Ozturk, MunirSoil samples and leaves of Origanum sipyleum L., were collected from 5 different altitudes of Spil mountain situated in the State of Manisa in the West Anatolian part of Turkey. The soils were analysed to determine pH, organic matter content, calcium carbonate and other chemical constituents. The photosynthetic pigment, proline and total protein contents of leaves were also determined. Results obtained showed that O. sipyleum grows on sandy-loam soils, rich in organic matter content, with pH varying between 6.11-6.97. It prefers slightly acidic and neutral soils, rich in N and P, but poor in K. The physiological analyses revealed that total protein and proline contents increased whereas photosynthetic pigment decreased at 520 and 790m altitudes. All parameters decreased at 1020 and 1150m altitudes. A statistically significant correlation was observed at higher altitudes.Öğe Variations in the proline and total protein contents in Origanum sipyleum L. from different altitudes of Spil mountain, Turkey(2013) Unal, Bengu Turkyilmaz; Guvensen, Aykut; Dereboylu, Aylin Esiz; Ozturk, MunirSoil samples and leaves of Origanum sipyleum L., were collected from 5 different altitudes of Spil mountain situated in the State of Manisa in the West Anatolian part of Turkey. The soils were analysed to determine pH, organic matter content, calcium carbonate and other chemical constituents. The photosynthetic pigment, proline and total protein contents of leaves were also determined. Results obtained showed that O. sipyleum grows on sandy-loam soils, rich in organic matter content, with pH varying between 6.11-6.97. It prefers slightly acidic and neutral soils, rich in N and P, but poor in K. The physiological analyses revealed that total protein and proline contents increased whereas photosynthetic pigment decreased at 520 and 790m altitudes. All parameters decreased at 1020 and 1150m altitudes. A statistically significant correlation was observed at higher altitudes.