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Does rhizospheric bacterium influence root exudation of sucrose? A preliminary investigation using Bacillus subtilis RR4
(Research Journal Biotechnology, 2018) Rekha, Kandaswamy; Sarbesh, Dongal D.; Usha, Balasundaram
Root exudation, the secretion of phytochemicals through roots, plays a critical role in regulating the microbial community in the plant rhizosphere. Sugars, particularly sucrose, comprise a major portion among the root exudates. Our aim was to demonstrate the effect of a rice rhizosphere isolate, Bacillus subtilis RR4, on the gene expression of sucrose transporters (OsSUT1-5) and root exudation of sucrose. Aseptically grown rice plants were treated with RR4 in a hydroponic setup for 48 h. Semi-quantitative reverse transcription-PCR was performed using RR4-treated rice roots to analyze the gene expression profile of the sucrose transporters (OsSUT1-5). HPLC analysis of the collected root exudates was performed to analyze the levels of sucrose exuded by the RR4-treated and untreated rice plants. Semi-quantitative RT-PCR analysis of sucrose transporter genes (OsSUT1-5) and HPLC analysis of root exudates revealed that B. subtilis RR4 did not influence the exudation of sucrose.